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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
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- Product number
- Ab128223 - Provider product page
- Provider
- Aladdin Scientific
- Product name
- Recombinant Smad4 Antibody
- Antibody type
- Recombinant
- Description
- Rabbit anti Human Smad4 Antibody, Recombinant, could be used for WB, ICC, IF and so on.Application:WB: 1/500-1/1000ICC/IF: 1/50-1/200Protein FunctionCommon SMAD (co-SMAD) is the coactivator and mediator of signal transduction by TGF-beta (transforming growth factor). Component of the heterotrimeric SMAD2/SMAD3-SMAD4 complex that forms in the nucleus and is required for the TGF-mediated signaling. Promotes binding of the SMAD2/SMAD4/FAST-1 complex to DNA and provides an activation function required for SMAD1 or SMAD2 to stimulate transcription. Component of the multimeric SMAD3/SMAD4/JUN/FOS complex which forms at the AP1 promoter site; required for syngernistic transcriptional activity in response to TGF-beta. May act as a tumor suppressor.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Antigen sequence
AA 316-552- Vial size
- 100_l,10_l,1ml,50_l
- Concentration
- 0,6 mg/ml
- Storage
- Store at 4¡C short term (1-2 weeks). Store at -20¡C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.
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Supportive validation
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- Recombinant Smad4 Antibody (Ab128223) - Western Blot All lanes: Recombinant Smad4 Antibody (Ab128223) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution Predicted band size: 60 kDa Observed band size: 63 kDa Exposure time: 4.3 s
Supportive validation
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- Recombinant Smad4 Antibody (Ab128223) - IF/ICC Immunocytochemistry analysis of Smad4 (green) in HeLa cells using Recombinant Smad4 Antibody (Ab128223), and cells were counterstained with DAPI (blue).
