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Western blot
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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Immunocytochemistry [2]
- Flow cytometry [1]
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- Product number
- MA5-15682 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SMAD4 Monoclonal Antibody (4G1C6)
- Antibody type
- Monoclonal
- Antigen
- Purifed from natural sources
- Description
- MA5-15682 targets SMAD4 in indirect ELISA, FACS, IF, IHC, and WB applications and shows reactivity with Human samples. The MA5-15682 immunogen is purified recombinant fragment of human SMAD4 expressed in E. Coli. . MA5-15682 detects SMAD4 which has a predicted molecular weight of approximately 65kDa.
- Reactivity
- Human, Mouse
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 4G1C6
- Vial size
- 100 µL
- Concentration
- Conc. Not Determined
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Operation of the Atypical Canonical Bone Morphogenetic Protein Signaling Pathway During Early Human Odontogenesis.
MiR-34a-3p alters proliferation and apoptosis of meningioma cells in vitro and is directly targeting SMAD4, FRAT1 and BCL2.
Hu X, Lin C, Ruan N, Huang Z, Zhang Y, Hu X
Frontiers in physiology 2022;13:823275
Frontiers in physiology 2022;13:823275
MiR-34a-3p alters proliferation and apoptosis of meningioma cells in vitro and is directly targeting SMAD4, FRAT1 and BCL2.
Werner TV, Hart M, Nickels R, Kim YJ, Menger MD, Bohle RM, Keller A, Ludwig N, Meese E
Aging 2017 Mar 23;9(3):932-954
Aging 2017 Mar 23;9(3):932-954
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of NIH/3T3 cells using SMAD4 monoclonal antibody (Product # MA5-15682) (Green). Blue: DRAQ5 fluorescent DNA dye. Red: actin filaments have been labeled with phalloidin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of NIH/3T3 cells using SMAD4 monoclonal antibody (Product # MA5-15682) (Green). Blue: DRAQ5 fluorescent DNA dye. Red: actin filaments have been labeled with phalloidin.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometric analysis of K562 cells using SMAD4 monoclonal antibody (Product # MA5-15682) (green) and negative control (purple).
