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Western blot
Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Blocking/Neutralizing [1]
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- Product number
- AF2296 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Goat Polyclonal Siglec-2/CD22 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects mouse Siglec-2/CD22 in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant human (rh) Siglec-2 is observed.
- Reactivity
- Mouse
- Host
- Goat
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references Enhancement and suppression of signaling by the conserved tail of IgG memory-type B cell antigen receptors.
Horikawa K, Martin SW, Pogue SL, Silver K, Peng K, Takatsu K, Goodnow CC
The Journal of experimental medicine 2007 Apr 16;204(4):759-69
The Journal of experimental medicine 2007 Apr 16;204(4):759-69
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
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- Experimental details
- Cell Adhesion Mediated by Siglec-2/CD22 and Neutral-ization by Mouse Siglec-2/ CD22 Antibody. Recombinant Mouse Siglec-2/ CD22 Fc Chimera (Catalog # 2296-SL), immobilized onto a microplate, supports the adhesion of human red blood cells in a dose-dependent manner (orange line). Adhesion elicited by Recombinant Mouse Siglec-2/ CD22 Fc Chimera (0.3 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse Siglec-2/CD22 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2296). The ND50 is typically 0.1-0.5 µg/mL.
