TA506407

antibody from OriGene

Targeting: CD22 SIGLEC-2, SIGLEC2

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

TA506407

Provider

OriGene

Proper citation

OriGene Cat#TA506407, RRID:AB_2623763

Product name

CD22 mouse monoclonal antibody, clone OTI1F12 (formerly 1F12)

Antibody type

Monoclonal

Description

CD22 mouse monoclonal antibody, clone OTI1F12 (formerly 1F12)

Reactivity

Canine

Host

Mouse

Conjugate

Unconjugated

Epitope

CD22

Isotype

IgG

Antibody clone number

OTI1F12

Vial size

100 µl

Concentration

1.00mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD22 (RC216939, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD22.

Validation comment

WB

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-CD22 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

Validation comment

WB

Immunocytochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Anti-CD22 mouse monoclonal antibody (TA506407) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CD22(RC216939).

Validation comment

IF

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunofluorescent staining of HeLa cells using anti-CD22 mouse monoclonal antibody (TA506407).

Validation comment

IF