TA506412

antibody from OriGene

Targeting: CD22 SIGLEC-2, SIGLEC2

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

TA506412

Provider

OriGene

Proper citation

OriGene Cat#TA506412, RRID:AB_2623764

Product name

CD22 mouse monoclonal antibody, clone OTI4C3 (formerly 4C3)

Antibody type

Monoclonal

Description

CD22 mouse monoclonal antibody, clone OTI4C3 (formerly 4C3)

Reactivity

Canine

Host

Mouse

Conjugate

Unconjugated

Epitope

CD22

Isotype

IgG

Antibody clone number

OTI4C3

Vial size

100 µl

Concentration

NULL

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD22 (RC216939, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD22.

Validation comment

WB

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-CD22 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

Validation comment

WB

Immunohistochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-CD22 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA506412)

Validation comment

IHC