Explore
Validate
Learn
Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Immunocytochemistry [1]
- Blocking/Neutralizing [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- AF2296 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse Siglec-2/CD22 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects mouse Siglec-2/CD22 in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant human (rh) Siglec-2 is observed.
- Reactivity
- Mouse
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
P35329- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Enhancement and suppression of signaling by the conserved tail of IgG memory-type B cell antigen receptors.
Horikawa K, Martin SW, Pogue SL, Silver K, Peng K, Takatsu K, Goodnow CC
The Journal of experimental medicine 2007 Apr 16;204(4):759-69
The Journal of experimental medicine 2007 Apr 16;204(4):759-69
No comments: Submit comment
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Siglec-2/CD22 in Mouse Splenocytes. Siglec-2/CD22 was detected in immersion fixed mouse splenocytes using Goat Anti-Mouse Siglec-2/CD22 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2296) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Cell Adhesion Mediated by Siglec-2/CD22 and Neutral-ization by Mouse Siglec-2/ CD22 Antibody. Recombinant Mouse Siglec-2/ CD22 Fc Chimera (Catalog # 2296-SL), immobilized onto a microplate, supports the adhesion of human red blood cells in a dose-dependent manner (orange line). Adhesion elicited by Recombinant Mouse Siglec-2/ CD22 Fc Chimera (0.3 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse Siglec-2/CD22 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2296). The ND50 is typically 0.1-0.5 µg/mL.
