NBP2-49890

antibody from Novus Biologicals
Targeting: CD22 SIGLEC-2, SIGLEC2
Provider product page for NBP2-49890
Immunocytochemistry
Flow cytometry

Antibody data

Product number
NBP2-49890 - Provider product page
Provider
Novus Biologicals
Product name
Mouse Monoclonal Siglec-2/CD22 Antibody
Antibody type
Monoclonal
Description
Protein A or G purified. The CD22 mAb (clone RFB4) recognizes surface CD22 expressed by mature peripheral B-cells and cytoplasmatic CD22 expressed by precursor B-cells. The sensitivity of RFB4 mAb is determined by staining well-defined blood samples from representative donors with serial-fold mAb dilutions to obtain a titration curve that allows relating the mAb concentration to the percentage of stained cells and geometric MFI (mean fluorescence intensity). For this purpose, a mAb-concentration range is selected to include both the saturation point (i.e. the mAb dilution expected to bind all epitopes on the target cell) and the detection threshold (i.e. the mAb dilution expected to represent the least amount of mAb needed to detect an identical percentage of cells). In practice, 50 ul of leukocytes containing 10^7 cells/ml are stained with 20 ul mAb of various dilutions to obtain a titration curve and to identify the saturation point and detection threshold. The final concentration of the product is then adjusted to be at least 3-fold above the detection threshold. In addition and to control lot-to-lot variation, the given lot is compared and adjusted to fluorescence standards with defined intensity.
Reactivity
Human
Host
Mouse
Isotype
IgG
Vial size
0.2 mg
Storage
Store at 4C in the dark.
CD22 protein structure - NBP2-49890 shown in red.