Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Flow cytometry [2]
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Validation data
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- Product number
- NBP2-49890PE - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal Siglec-2/CD22 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified. The CD22 mAb (clone RFB4) recognizes surface CD22 expressed by mature peripheral B-cells and cytoplasmatic CD22 expressed by precursor B-cells. The sensitivity of RFB4 mAb is determined by staining well-defined blood samples from representative donors with serial-fold mAb dilutions to obtain a titration curve that allows relating the mAb concentration to the percentage of stained cells and geometric MFI (mean fluorescence intensity).For this purpose, a mAb-concentration range is selected to include both the saturation point (i.e. the mAb dilution expected to bind all epitopes on the target cell) and the detection threshold (i.e. the mAb dilution expected to represent the least amount of mAb needed to detect an identical percentage of cells). In practice, 50 ul of leukocytes containing 10^7 cells/ml are stained with 20 ul mAb of various dilutions to obtain a titration curve and to identify the saturation point and detection threshold. The final concentration of the product is then adjusted to be at least 3-fold above the detection threshold. In addition and to control lot-to-lot variation, the given lot is compared and adjusted to fluorescence standards with defined intensity.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Yellow dye
- Isotype
- IgG
- Vial size
- 2 ml
- Storage
- Store at 4C in the dark.
Submitted references MicroRNA profiling in canine multicentric lymphoma.
Craig KKL, Wood GA, Keller SM, Mutsaers AJ, Wood RD
PloS one 2019;14(12):e0226357
PloS one 2019;14(12):e0226357
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: Siglec-2/CD22 Antibody (RFB4) [PE] [NBP2-49890PE] - Flow cytometric analysis of a normal blood sample after immunostaining with Siglec-2/CD22 Antibody (RFB4) [PE].
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: Siglec-2/CD22 Antibody (RFB4) [PE] [NBP2-49890PE] - Flow cytometric analysis of a normal blood sample after immunostaining with Siglec-2/CD22 Antibody (RFB4) [PE].