17-4229-42

antibody from Invitrogen Antibodies
Targeting: CD22 SIGLEC-2, SIGLEC2
Provider product page for 17-4229-42

Antibody data

Product number
17-4229-42 - Provider product page
Provider
Invitrogen Antibodies
Product name
CD22 Monoclonal Antibody (S-HCL-1), APC, eBioscience™
Antibody type
Monoclonal
Antigen
Other
Description
Description: The S-HCl-1 monoclonal antibody recognizes human CD22 (Siglec-2), which is a member of the siglec subgroup of the Ig superfamily. CD22 is a type I transmembrane glycoprotein composed of two polypeptide chains, CD22a and CD22β, of 130 and 140 kDa respectively, produced by alternative splicing of the CD22 gene. CD22 is expressed at high levels on mature B cells and B cell lymphomas. The extracellular portion of CD22 contains seven Ig-like domains, some of which are capable of binding ligands with sialic acid moieties expressed on epithelial, endothelial, B and T cells. The intracellular portion of CD22 contains 6 tyrosine residues contained within immunotyrosine-based inhibitory motifs (ITIM) and immunotyrosine-based activation-like motifs, which are phosphorylated upon B-cell receptor engagement, which enables CD22 to participate in the positive and negative regulation of B-cell receptor signaling. Applications Reported: This S-HCL-1 antibody has been reported for use in flow cytometric analysis. Applications Tested: This S-HCL-1 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser. Filtration: 0.2 µm post-manufacturing filtered.
Reactivity
Human
Host
Mouse
Isotype
IgG
Antibody clone number
S-HCL-1
Vial size
100 Tests
Concentration
5 µL/Test
Storage
4° C, store in dark, DO NOT FREEZE!
CD22 protein structure - 17-4229-42 shown in red.