933-MSM5-P1

antibody from Invitrogen Antibodies

Targeting: CD22 SIGLEC-2, SIGLEC2

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

933-MSM5-P1

Provider

Invitrogen Antibodies

Product name

CD22/BL-CAM Monoclonal Antibody (BLCAM, 1795)

Antibody type

Monoclonal

Antigen

Recombinant protein fragment

Description

Immunohistochemistry (PFA fixed): incubate antibody for 30 min RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 degrees C followed by cooling at RT for 20 minutes.

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

BLCAM, 1795

Vial size

100 µg

Concentration

200 µg/mL

Storage

4° C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of Raji cell lysate using CD22, BL-CAM Monoclonal Antibody (Product # 933-MSM5-P1).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of Raji cell lysate using CD22, BL-CAM Monoclonal Antibody (Product # 933-MSM5-P1).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of Raji and Ramos cell lysates using CD22, BL-CAM Monoclonal Antibody (Product # 933-MSM5-P1).

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry/Immunofluorescence analysis of paraformaldehyde-fixed Ramos cells using CD22, BL-CAM Monoclonal Antibody (Product # 933-MSM5-P1) followed by goat anti-Mouse IgG conjugated to CF488 (green). Nuclei are stained with Reddot..

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry (Paraffin) analysis performed in formalin fixed, paraffin-embedded human spleen using CD22, BL-CAM Monoclonal Antibody (Product # 933-MSM5-P1).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry (Paraffin) analysis performed in formalin fixed, paraffin-embedded human tonsil using CD22, BL-CAM Monoclonal Antibody (Product # 933-MSM5-P1).

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow Cytometry analysis of paraformaldehyde-fixed MOLT4 cells using CD22, BL-CAM Monoclonal Antibody (Product # 933-MSM5-P1) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Analysis of Protein Array containing more than 19,000 full-length human proteins CD22, BL-CAM Monoclonal Antibody (Product # 933-MSM5-P1). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

SDS-PAGE Analyis Purified CD22, BL-CAM Monoclonal Antibody (Product # 933-MSM5-P1). Confirmation of Purity and Integrity of Antibody.