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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
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Validation data
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- Product number
- MAB7290 - Provider product page
- Provider
- R&D Systems
- Product name
- Human Phospho-Siglec-2/CD22 (Y822) Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human CD22 when phosphorylated at Y822 in direct ELISAs.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
P20273- Isotype
- IgG
- Antibody clone number
- 726830
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human Siglec-2/CD22 by Western Blot. Western blot shows lysates of Ramos human Burkitt's lymphoma cell line and Raji human Burkitt's lymphoma cell line untreated (-) or treated (+) with 1 mM Pervanadate (PV) for 30 minutes. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Phospho-Siglec-2/CD22 (Y822) Monoclonal Antibody (Catalog # MAB7290) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Siglec-2/CD22 at approximately 140 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Phospho-Siglec-2/CD22 (Y822) in Raji Human Cell Line. Siglec-2/CD22 phosphorylated at Y822 was detected in immersion fixed Raji human Burkitt's lymphoma cell line unstimulated (lower panel) or stimulated (upper panel) with Pervanadate using Mouse Anti-Human Phospho-Siglec-2/CD22 (Y822) Monoclonal Antibody (Catalog # MAB7290) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
