NB100-64761

antibody from Novus Biologicals

Targeting: IL2RA CD25, IDDM10, IL2R

ELISA (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

NB100-64761

Provider

Novus Biologicals

Proper citation

Novus Cat#NB100-64761, RRID:AB_2125467

Product name

Mouse Monoclonal CD25/IL-2R alpha Antibody

Antibody type

Monoclonal

Description

Protein G purified. NB100-64761 recognizes the alpha chain of rat CD25, otherwise known as IL-2 receptor alpha, a 55kDa type I membrane glycoprotein, expressed by activated T cells but not resting lymphocytes. CD25 is also expressed by dendritic cells found in the thymus medulla. *This clone has been described reacting with paraffin-embedded material following PLP fixation (periodate-lysine-paraformaldehyde) ^(6). Clone OX-39 has been shown to weakly inhibit the binding of IL-2 to Con-A stimulated spleen blasts.

Reactivity

Rat

Host

Mouse

Isotype

IgG

Vial size

0.25 mg

Concentration

1.0 mg/ml

Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

References

Submitted references

Enrichment of early fetal-liver hemopoietic stem cells of the rat using monoclonal antibodies against the transferrin receptor, Thy-1, and MRC-OX82.

Crook K, Hunt SV
Developmental immunology 1996;4(4):235-46

---

Enrichment of early fetal-liver hemopoietic stem cells of the rat using monoclonal antibodies against the transferrin receptor, Thy-1, and MRC-OX82.

Crook K, Hunt SV
Developmental immunology 1996;4(4):235-46

---

Vascular thymus transplantation in rats. Technique, morphology, and function.

Kampinga J, Schuurman HJ, Pol GH, Bartels H, Broekhuizen R, Vaessen LM, Tielen FJ, Rozing J, Blaauw EH, Roser B
Transplantation 1990 Oct;50(4):669-78

---

Vascular thymus transplantation in rats. Technique, morphology, and function.

Kampinga J, Schuurman HJ, Pol GH, Bartels H, Broekhuizen R, Vaessen LM, Tielen FJ, Rozing J, Blaauw EH, Roser B
Transplantation 1990 Oct;50(4):669-78

---

T-cell lymphoma lines derived from rat thymomas induced by Moloney murine leukemia virus: phenotypic diversity and its implications.

Lazo PA, Klein-Szanto AJ, Tsichlis PN
Journal of virology 1990 Aug;64(8):3948-59

---

T-cell lymphoma lines derived from rat thymomas induced by Moloney murine leukemia virus: phenotypic diversity and its implications.

Lazo PA, Klein-Szanto AJ, Tsichlis PN
Journal of virology 1990 Aug;64(8):3948-59

---

Interleukin 2 receptor in rat heart allograft rejection.

Bouchot O, Anegon I, Romaniuk A, Jacques Y, Paineau J, Soulillou JP
Transplantation 1989 Dec;48(6):918-22

---

Interleukin 2 receptor in rat heart allograft rejection.

Bouchot O, Anegon I, Romaniuk A, Jacques Y, Paineau J, Soulillou JP
Transplantation 1989 Dec;48(6):918-22

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: CD25/IL-2 R alpha Antibody (OX-39) [NB100-64761] - Rat lymph node cryosection with Mouse anti Rat CD25 followed by horseradish peroxidase conjugated Goat anti Mouse IgG1 as a detection reagent. Medium power

Flow cytometry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow Cytometry: CD25/IL-2 R alpha Antibody (OX-39) [NB100-64761] - Staining of Con A activated rat spleen cells with Mouse anti Rat CD25: Biotin