Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Flow cytometry [1]
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Validation data
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- Product number
- MA1-19233 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD25 Monoclonal Antibody (MEM-140)
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- This antibody reacts with an extracellular epitope of CD25 (Interleukin-2 receptor alpha chain), a 55 kDa type I transmembrane glycoprotein expressed on activated B and T lymphocytes, activated monocytes/macrophages and on CD4+ T lymphocytes (T regulatory cells); it is lost on resting B and T lymphocytes.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgM
- Antibody clone number
- MEM-140
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- 4° C, do not freeze
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Flow cytometry surface staining pattern of human PHA stimulated peripheral human blood mononclear cells stained using anti-human CD25 (MEM-140) purified Monoclonal antibody (Product # MA1-19233) (concentration in sample 0.6 µg/mL, GAM APC, red) and human PHA stimulated peripheral blood mononclear cells unstained by primary antibody (GAM APC, blue).