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ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunohistochemistry [1]
- Blocking/Neutralizing [1]
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- Product number
- AF2438 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Goat Polyclonal CD25/IL-2R alpha Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects mouse CD25/IL-2 R alpha in ELISAs and Western blots. In sandwich ELISAs, less than 0.1% cross-reactivity with recombinant human CD25/IL-2 R alpha is observed.
- Reactivity
- Mouse
- Host
- Goat
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references CCL28 promotes locomotor recovery after spinal cord injury via recruiting regulatory T cells.
Wang P, Qi X, Xu G, Liu J, Guo J, Li X, Ma X, Sun H
Aging 2019 Sep 26;11(18):7402-7415
Aging 2019 Sep 26;11(18):7402-7415
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- CD25/IL-2 R alpha in Mouse Spleen. CD25/IL-2 R alpha was detected in immersion fixed frozen sections of mouse spleen using 5 µg/mL Goat Anti-Mouse CD25/IL-2 R alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2438) overnight at 4 °C. Tissue was stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained (green). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Cell Proliferation Induced by IL-2 and Neutralization by Mouse CD25IL-2 R alpha Antibody. Recombinant Mouse IL-2 (Catalog # 402-ML) stimulates proliferation in the CTLL-2 mouse cytotoxic T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL-2 (2 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse CD25/IL-2 R alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2438). The ND50 is typically 5-20 µg/mL.
