Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Flow cytometry [1]
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Validation data
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- Product number
- FAB1020A-100 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal CD25/IL-2R alpha Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human CD25/IL-2 R alpha in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) IL-2 R gamma or rhIL-15 R alpha is observed.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Red dye
- Isotype
- IgG
- Vial size
- 100 Tests
- Storage
- Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 degreesC as supplied.
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Detection of CD25/IL-2 R alpha in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) treated with 20 ng/mL Recombinant Human IL-2 (Catalog # 202-IL) and 10 ng/mL Recombinant Human TGF-beta 1 (Catalog # 240-B) for 2 days were stained with Mouse Anti-Human CD4 PE-conjugated Monoclonal Antibody (Catalog # FAB3791P) and either (A) Mouse Anti-Human CD25/IL-2 R alpha APC-conjugated Monoclonal Antibody (Catalog # FAB1020A) or (B) Mouse IgG2A Allophycocyanin Isotype Control (Catalog # IC003A). View our protocol for Staining Membrane-associated Proteins.