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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- ELISA [1]
- Immunohistochemistry [2]
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- Product number
- NBP2-37349 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal CD25/IL-2R alpha Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified.
- Reactivity
- Human, Mouse, Rat, Simian
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 1 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: CD25/IL-2R alpha Antibody (1B5D12) [NBP2-37349] - Analysis using IL2RA mouse mAb against Hela (1), MOLT4 (2), HEK293 (3), A549 (4), Jurkat (5), K562 (6), Cos7 (7), PC-12 (8) and NIH/3T3 (9) cell lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: CD25/IL-2R alpha Antibody (1B5D12) [NBP2-37349] - Analysis using IL2RA mAb against human IL2RA recombinant protein. (Expected MW is 37.5 kDa)
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: CD25/IL-2R alpha Antibody (1B5D12) [NBP2-37349] - Analysis using IL2RA mAb against HEK293 (1) and IL2RA (AA: 34-139)-hIgGFc transfected HEK293 (2) cell lysate.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: CD25/IL-2R alpha Antibody (1B5D12) [NBP2-37349] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: CD25/IL-2R alpha Antibody (1B5D12) [NBP2-37349] - Analysis of rectum cancer tissues using IL2RA mouse mAb with DAB staining.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: CD25/IL-2R alpha Antibody (1B5D12) [NBP2-37349] - Analysis of bladder cancer tissues using IL2RA mouse mAb with DAB staining.
