Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Flow cytometry [1]
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Validation data
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- Product number
- A15802 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD25 Monoclonal Antibody (MEM-181), PerCP
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- This antibody reacts with an extracellular epitope of CD25 (Interleukin-2 receptor alpha chain), a 55 kDa type I transmembrane glycoprotein expressed on activated B and T lymphocytes, activated monocytes/macrophages and on CD4+ T lymphocytes (T regulatory cells); it is lost on resting B and T lymphocytes.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Red dye
- Isotype
- IgG
- Antibody clone number
- MEM-181
- Vial size
- 100 Tests
- Storage
- 4° C, store in dark, DO NOT FREEZE!
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Separation of CD25 positive cells stained using anti-human CD25 (MEM-181) PerCP Monoclonal antibody (Product # A15802) (10 µL reagent per million cells in 100 µL of cell suspension, red-filled) from cells stained using mouse IgG1 isotype control (MOPC-21) PerCP antibody (concentration in sample 3 µg/mL, same as CD25 PerCP concentration, black-dashed) in flow cytometry analysis (surface staining) of human PHA stimulated peripheral human blood mononuclear cells.
- Conjugate
- Red dye