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Western blot
Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
- References [4]
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- Validations
- Blocking/Neutralizing [1]
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- Product number
- MAB1020 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal CD25/IL-2R alpha Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human CD25/IL-2 R alpha in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) IL-2 R gamma or rhIL-15 R alpha is observed.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 500 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references E-cadherin phosphorylation occurs during its biosynthesis to promote its cell surface stability and adhesion.
A novel human CD4+ T-cell inducer subset with potent immunostimulatory properties.
Human intestinal intraepithelial lymphocytes keep TNF alpha levels low by cell uptake and feedback inhibition of transcription.
Functional characterization of EBV-encoded nuclear antigen 1-specific CD4+ helper and regulatory T cells elicited by in vitro peptide stimulation.
McEwen AE, Maher MT, Mo R, Gottardi CJ
Molecular biology of the cell 2014 Aug 15;25(16):2365-74
Molecular biology of the cell 2014 Aug 15;25(16):2365-74
A novel human CD4+ T-cell inducer subset with potent immunostimulatory properties.
Ndhlovu LC, Leal FE, Eccles-James IG, Jha AR, Lanteri M, Norris PJ, Barbour JD, Wachter DJ, Andersson J, Taskén K, Torheim EA, Aandahl EM, Kallas EG, Nixon DF
European journal of immunology 2010 Jan;40(1):134-41
European journal of immunology 2010 Jan;40(1):134-41
Human intestinal intraepithelial lymphocytes keep TNF alpha levels low by cell uptake and feedback inhibition of transcription.
Ebert EC, Mehta V
Cellular immunology 2006 May;241(1):7-13
Cellular immunology 2006 May;241(1):7-13
Functional characterization of EBV-encoded nuclear antigen 1-specific CD4+ helper and regulatory T cells elicited by in vitro peptide stimulation.
Voo KS, Peng G, Guo Z, Fu T, Li Y, Frappier L, Wang RF
Cancer research 2005 Feb 15;65(4):1577-86
Cancer research 2005 Feb 15;65(4):1577-86
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- IL-2 binding to Human CD25/IL-2R alpha-transfected HEK293 Human Cell Line is Blocked by Human CD25/IL-2R alpha Antibody. IL-2 binding to Human CD25/IL-2R alpha-transfected HEK293 Human Cell Line is Blocked by Human CD25/IL-2R alpha Antibody.
