MAB223-100

antibody from Novus Biologicals

Targeting: IL2RA CD25, IDDM10, IL2R

Western blot (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Blocking/Neutralizing (Supportive data in Antibodypedia)

Antibody Data

Product number

MAB223-100

Provider

Novus Biologicals

Product name

Mouse Monoclonal CD25/IL-2R alpha Antibody

Antibody type

Monoclonal

Description

Protein A or G purified from ascites. Detects human CD25/IL-2 R alpha in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 4% cross-reactivity with recombinant human (rh) IL-2 R beta is observed and no cross-reactivity with rhIL-2 R gamma , rhIL-1 RI, rhIL-1 RII, rhIL-4 R, rhIL-5 R alpha , rhIL-6 R, rhIL-7 R, rhIL-9 R, and rhIL-10 R is observed.

Reactivity

Human

Host

Mouse

Isotype

IgG

Vial size

100 ug

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

Blocking/Neutralizing

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Cell Proliferation Induced by IL-2 and Neutralization by Human CD25/IL-2 R alpha Antibody. Recombinant Human IL-2 (Catalog # 202-IL) stimulates proliferation in the N1186 human T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-2 (1 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human CD25/IL-2 R alpha Monoclonal Antibody (Catalog # MAB223). The ND₅₀ is typically 0.5-1.0 µg/mL.