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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Immunocytochemistry [1]
- Blocking/Neutralizing [1]
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- Product number
- MAB223-100 - Provider product page
- Provider
- R&D Systems
- Product name
- Human CD25/IL-2R alpha Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from ascites. Detects human CD25/IL-2 R alpha in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 4% cross-reactivity with recombinant human (rh) IL-2 R beta is observed and no cross-reactivity with rhIL-2 R gamma , rhIL-1 RI, rhIL-1 RII, rhIL-4 R, rhIL-5 R alpha , rhIL-6 R, rhIL-7 R, rhIL-9 R, and rhIL-10 R is observed.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
P01589- Isotype
- IgG
- Antibody clone number
- 22722
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Immune Checkpoint Blockade Restores HIV-Specific CD4 T Cell Help for NK Cells.
Enhanced immune response of MAIT cells in tuberculous pleural effusions depends on cytokine signaling.
Porichis F, Hart MG, Massa A, Everett HL, Morou A, Richard J, Brassard N, Veillette M, Hassan M, Ly NL, Routy JP, Freeman GJ, Dubé M, Finzi A, Kaufmann DE
Journal of immunology (Baltimore, Md. : 1950) 2018 Aug 1;201(3):971-981
Journal of immunology (Baltimore, Md. : 1950) 2018 Aug 1;201(3):971-981
Enhanced immune response of MAIT cells in tuberculous pleural effusions depends on cytokine signaling.
Jiang J, Chen X, An H, Yang B, Zhang F, Cheng X
Scientific reports 2016 Sep 2;6:32320
Scientific reports 2016 Sep 2;6:32320
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- CD25/IL-2 R alpha in Human PBMCs. CD25/IL-2 R alpha was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) stimulated with PHA and monensin using Mouse Anti-Human CD25/IL-2 R alpha Monoclonal Antibody (Catalog # MAB223) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (yellow; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Cell Proliferation Induced by IL-2 and Neutralization by Human CD25/IL-2 R alpha Antibody. Recombinant Human IL-2 (Catalog # 202-IL) stimulates proliferation in the N1186 human T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-2 (1 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human CD25/IL-2 R alpha Monoclonal Antibody (Catalog # MAB223). The ND50 is typically 0.5-1.0 µg/mL.
