Antibody data
- Antibody Data
- Antigen structure
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- Validations
- Flow cytometry [3]
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- Product number
- NBP2-52660-0.2mg - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rabbit Monoclonal CD25/IL-2R alpha Antibody
- Antibody type
- Monoclonal
- Description
- Protein A purified. Binds to p55 chain of IL2R on human T cells, Tac antigen, which is expressed on T cells activated by mitogens, soluble antigens, and alloantigens but not on resting T cells, thymocytes, B cells, monocytes, activated B cells, leukemic T cell blasts from patients with acute lymphoblastic leukemia, and long-term T cell lines.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.2 mg
- Storage
- Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: CD25/IL-2 R alpha Antibody (Daclizumab) [NBP2-52660] - Cynomolgus monkey lymphocytes were stained with an isotype control (red) or the rabbit-chimeric version of Daclizumab (NBP2-52660, blue) at a concentration of 1 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody (ab150073) and cells analysed on a FlowJo single-cell flow cytometer.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: CD25/IL-2R alpha Antibody (Daclizumab) [NBP2-52660] - CD25/IL-2 R alpha Antibody (Daclizumab) [NBP2-52660] - Rhesus monkey lymphocytes were stained with an isotype control (red) or the rabbit-chimeric version of Daclizumab (NBP2-52660, blue) at a concentration of 1 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody (ab150073) and cells analysed on a FlowJo single-cell flow cytometer.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: CD25/IL-2R alpha Antibody (Daclizumab) [NBP2-52660] - CD25/IL-2 R alpha Antibody (Daclizumab) [NBP2-52660] - Human lymphocytes were stained with an isotype control (panel A) or the rabbit-chimeric version of Daclizumab (NBP2-52660, panel B) at a concentration of 1 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.