Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [3]
Submit
Validation data
Reference
Comment
Report error
- Product number
- NBP2-52661-0.2mg - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rabbit Monoclonal CD25/IL-2R alpha Antibody
- Antibody type
- Monoclonal
- Description
- Protein A purified. Basiliximab recognises the human IL-2R and binds to the epitope (116)ERIYHFV(122) within the extracellular domain of the CD25 subunit -binding is abolished when 2 or more residues within the epitope are mutated. This epitope overlaps with the interaction site of CD25 and IL-2. CD25 is found in the high-affinity IL-2R ((alpha)(beta)(gamma)c) -T cells only express CD25 upon activation. IL-2 binding to IL-2R on T cells modulates proliferation and differentiation.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.2 mg
- Storage
- Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: CD25/IL-2 R alpha Antibody (Basiliximab) [NBP2-52661] - Staining of Human thyroid cancer (lane 1), rat spleen (lane 2), mouse lymph node (lane 3), mouse thymus (lane 4) and rat small intestines (lane 5)samples were resolved on a 10% SDS PAGE gel and blots probed at 1 ug/ml before being detected by a secondary antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunofluorescence: CD25/IL-2 R alpha Antibody (Basiliximab) [NBP2-52661] - Formaldehyde-fixed rat thymus slices were stained at 5 ug/ml and detected with a FITC-conjugated secondary antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: CD25/IL-2 R alpha Antibody (Basiliximab) [NBP2-52661] - Staining of rat spleens using anti-CD25 antibody. Formalin fixed rat spleen slices were were stained at 5 ug/ml. The anti-human CD25 antibody also shows some cross-reactivity with rat in IHC.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: CD25/IL-2 R alpha Antibody (Basiliximab) [NBP2-52661] - Flow-cytometry using anti-CD25 antibody Basiliximab (NBP2-52661) Cynomolgus monkey lymphocytes were stained with an isotype control (panel A) or the rabbit-chimeric version of Basiliximab (panel B) at a concentration of 1 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: CD25/IL-2 R alpha Antibody (Basiliximab) [NBP2-52661] - Flow-cytometry using the anti-CD25 (IL2R) research biosimilar antibody Basiliximab. Human lymphocytes were stained with an isotype control (panel A) or the rabbit-chimeric version of Basiliximab (panel B) at a concentration of 1 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: CD25/IL-2 R alpha Antibody (Basiliximab) [NBP2-52661] - Flow-cytometry using anti-CD25 antibody Basiliximab (NBP2-52661) Rhesus monkey lymphocytes were stained with an isotype control (panel A) or the rabbit-chimeric version of Basiliximab (panel B) at a concentration of 1 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.