NBP2-27425-0.5mg

antibody from Novus Biologicals

Targeting: IL2RA CD25, IDDM10, IL2R

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

NBP2-27425-0.5mg

Provider

Novus Biologicals

Product name

Rat Monoclonal CD25/IL-2 R alpha Antibody

Antibody type

Monoclonal

Description

Protein G purified.

Reactivity

Mouse

Host

Rat

Isotype

IgG

Vial size

0.5 mg

Concentration

1 mg/ml

Storage

Store at -20C. Avoid freeze-thaw cycles.

Flow cytometry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow Cytometry: CD25/IL-2 R alpha Antibody (PC61) [NBP2-27425] - Analysis using the Allophycocyanin conjugate of NBP2-27425. Staining of IL2RA/CD25 in ConA stimulated mouse splenocytes using 0.1 ug/1x10^6 cells of NBP2-30135. Shaded histogram represents cells without antibody; green represents isotype control (ebioscience); red represents anti-IL2RA/CD25 antibody.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow (Cell Surface): CD25/IL-2 R alpha Antibody (PC61) [NBP2-27425] - Analysis using the PE conjugate of NBP2-27425. Staining of CD25 in BALB/c mouse splenocytes. Cells were stained with anti-CD4 FITC this antibody and either 1 ug of isotype control antibody (left, ) or 1 ug of anti-CD25 PE (right).

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow Cytometry: CD25/IL-2 R alpha Antibody (PC61) [NBP2-27425] - analysis of CD25 in ConA stimulated mouse splenocytes using 0.1 ug of CD25 antibody. Shaded histogram represents cells without antibody; green represents isotype control; purple represents CD25 antibody.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow Cytometry: CD25/IL-2 R alpha Antibody (PC61) [NBP2-27425] - Analysis using the FITC conjugate of NBP2-27425. Staining of CD4 and CD25 on mouse splenocytes using 1 ug/1x10^6 cells of CD4 and CD25 antibody.