Antibody data
- Antibody Data
- Antigen structure
- References [47]
- Comments [0]
- Validations
- Flow cytometry [3]
- Other assay [6]
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- Product number
- 12-0259-41 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD25 Monoclonal Antibody (BC96), PE, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The BC96 monoclonal antibody reacts with human CD25, the 55 kDa interleukin-2 receptor alpha chain (IL-2Ralpha). CD25 is expressed by early progenitors of T and B lineage as well as by activated mature T and B lymphocytes. By itself, CD25 binds IL-2 only with low affinity. However, CD25 associates with CD122 (IL-2 receptor beta chain) and CD132 (common gamma chain) to form the high affinity IL-2 receptor. CD25 plays a role in lymphocyte differentiating and activation/proliferation. Applications Reported:The BC96 antibody has been reported for use in flow cytometric analysis. Applications Tested: Has been pre-titrated and tested and can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Yellow dye
- Isotype
- IgG
- Antibody clone number
- BC96
- Vial size
- 25 Tests
- Concentration
- 5 µL/Test
- Storage
- 4° C, store in dark, DO NOT FREEZE!
Submitted references The Composition of Adipose-Derived Regenerative Cells Isolated from Lipoaspirate Using a Point of Care System Does Not Depend on the Subject's Individual Age, Sex, Body Mass Index and Ethnicity.
Dysregulated Peripheral Invariant Natural Killer T Cells in Plaque Psoriasis Patients.
CAR T cells targeting tumor-associated exons of glypican 2 regress neuroblastoma in mice.
Mucosal-associated invariant T cell responses differ by sex in COVID-19.
T cell receptor-dependent S-acylation of ZAP-70 controls activation of T cells.
MondoA-Thioredoxin-Interacting Protein Axis Maintains Regulatory T-Cell Identity and Function in Colorectal Cancer Microenvironment.
Th2 Biased Immunity With Altered B Cell Profiles in Circulation of Patients With Sporotrichosis Caused by Sporothrix globosa.
Th17 reprogramming of T cells in systemic juvenile idiopathic arthritis.
Human Fc Receptor-like 3 Inhibits Regulatory T Cell Function and Binds Secretory IgA.
Epigenetically modulated FOXM1 suppresses dendritic cell maturation in pancreatic cancer and colon cancer.
Disruption of PD-1 Enhanced the Anti-tumor Activity of Chimeric Antigen Receptor T Cells Against Hepatocellular Carcinoma.
Functionalization-dependent effects of cellulose nanofibrils on tolerogenic mechanisms of human dendritic cells.
The Effects of High Mobility Group Box-1 Protein on Peripheral Treg/Th17 Balance in Patients with Atherosclerosis.
MicroRNA-31 Targets Thymic Stromal Lymphopoietin in Mucosal Infiltrated CD4+ T Cells: A Role in Achieving Mucosal Healing in Ulcerative Colitis?
MicroRNA-210 overexpression promotes psoriasis-like inflammation by inducing Th1 and Th17 cell differentiation.
CRISPR-Cas9-mediated disruption of PD-1 on human T cells for adoptive cellular therapies of EBV positive gastric cancer.
Landscape of Infiltrating T Cells in Liver Cancer Revealed by Single-Cell Sequencing.
Combinations of isoform-targeted histone deacetylase inhibitors and bryostatin analogues display remarkable potency to activate latent HIV without global T-cell activation.
Chemical proteomic map of dimethyl fumarate-sensitive cysteines in primary human T cells.
Equilibrium of Treg/Th17 cells of peripheral blood in syphilitic patients with sero-resistance.
Regulatory T Cell Modulation by CBP/EP300 Bromodomain Inhibition.
CRISPR-Cas9 mediated efficient PD-1 disruption on human primary T cells from cancer patients.
Human Head and Neck Squamous Cell Carcinoma-Associated Semaphorin 4D Induces Expansion of Myeloid-Derived Suppressor Cells.
Alloantigen-specific regulatory T cells generated with a chimeric antigen receptor.
Severe impairment of regulatory T-cells and Th1-lymphocyte polarization in patients with Gaucher disease.
Age-Associated Differences in MiRNA Signatures Are Restricted to CD45RO Negative T Cells and Are Associated with Changes in the Cellular Composition, Activation and Cellular Ageing.
Impact of Pre-Transplant Anti-T Cell Globulin (ATG) on Immune Recovery after Myeloablative Allogeneic Peripheral Blood Stem Cell Transplantation.
Regulatory Dendritic Cells Restrain NK Cell IFN-γ Production through Mechanisms Involving NKp46, IL-10, and MHC Class I-Specific Inhibitory Receptors.
Fine specificities of natural regulatory T cells after IVIG therapy in patients with Kawasaki disease.
Safety and immunologic correlates of Melanoma GVAX, a GM-CSF secreting allogeneic melanoma cell vaccine administered in the adjuvant setting.
GITR engagement in combination with CTLA-4 blockade completely abrogates immunosuppression mediated by human liver tumor-derived regulatory T cells ex vivo.
An increased abundance of tumor-infiltrating regulatory T cells is correlated with the progression and prognosis of pancreatic ductal adenocarcinoma.
Immune responses induced by T-cell vaccination in patients with rheumatoid arthritis.
Equisetum arvense (common horsetail) modulates the function of inflammatory immunocompetent cells.
Neuropilin-1 expression characterizes T follicular helper (Tfh) cells activated during B cell differentiation in human secondary lymphoid organs.
Small molecule inhibitor of antigen binding and presentation by HLA-DR2b as a therapeutic strategy for the treatment of multiple sclerosis.
Calcineurin inhibitor treatment of intravenous immunoglobulin-resistant Kawasaki disease.
Inhibition of SLPI ameliorates disease activity in experimental autoimmune encephalomyelitis.
Histone deacetylase inhibitors impair NK cell viability and effector functions through inhibition of activation and receptor expression.
Immunophenotyping at the time of diagnosis distinguishes two groups of nasopharyngeal carcinoma patients: implications for adoptive immunotherapy.
Immunophenotyping at the time of diagnosis distinguishes two groups of nasopharyngeal carcinoma patients: implications for adoptive immunotherapy.
Angiopoietin 2 stimulates TIE2-expressing monocytes to suppress T cell activation and to promote regulatory T cell expansion.
Flt3-L increases CD4+CD25+Foxp3+ICOS+ cells in the lungs of cockroach-sensitized and -challenged mice.
Fc receptor-like 3 protein expressed on IL-2 nonresponsive subset of human regulatory T cells.
T-cell responses to survivin in cancer patients undergoing radiation therapy.
T regulatory cell levels decrease in people infected with Schistosoma mansoni on effective treatment.
Functional inactivation of EBV-specific T-lymphocytes in nasopharyngeal carcinoma: implications for tumor immunotherapy.
Schmitz C, Alt C, Azares AR, Pearce DA, Facile TR, Furia JP, Maffulli N, Huang C, Alt EU
Cells 2022 Dec 21;12(1)
Cells 2022 Dec 21;12(1)
Dysregulated Peripheral Invariant Natural Killer T Cells in Plaque Psoriasis Patients.
Hu Y, Chen Y, Chen Z, Zhang X, Guo C, Yu Z, Xu P, Sun L, Zhou X, Gong Y, Yu Q, Shi Y
Frontiers in cell and developmental biology 2021;9:799560
Frontiers in cell and developmental biology 2021;9:799560
CAR T cells targeting tumor-associated exons of glypican 2 regress neuroblastoma in mice.
Li N, Torres MB, Spetz MR, Wang R, Peng L, Tian M, Dower CM, Nguyen R, Sun M, Tai CH, de Val N, Cachau R, Wu X, Hewitt SM, Kaplan RN, Khan J, St Croix B, Thiele CJ, Ho M
Cell reports. Medicine 2021 Jun 15;2(6):100297
Cell reports. Medicine 2021 Jun 15;2(6):100297
Mucosal-associated invariant T cell responses differ by sex in COVID-19.
Yu C, Littleton S, Giroux NS, Mathew R, Ding S, Kalnitsky J, Yang Y, Petzold E, Chung HA, Rivera GO, Rotstein T, Xi R, Ko ER, Tsalik EL, Sempowski GD, Denny TN, Burke TW, McClain MT, Woods CW, Shen X, Saban DR
Med (New York, N.Y.) 2021 Jun 11;2(6):755-772.e5
Med (New York, N.Y.) 2021 Jun 11;2(6):755-772.e5
T cell receptor-dependent S-acylation of ZAP-70 controls activation of T cells.
Tewari R, Shayahati B, Fan Y, Akimzhanov AM
The Journal of biological chemistry 2021 Jan-Jun;296:100311
The Journal of biological chemistry 2021 Jan-Jun;296:100311
MondoA-Thioredoxin-Interacting Protein Axis Maintains Regulatory T-Cell Identity and Function in Colorectal Cancer Microenvironment.
Lu Y, Li Y, Liu Q, Tian N, Du P, Zhu F, Han Y, Liu X, Liu X, Peng X, Wang X, Wu Y, Tong L, Li Y, Zhu Y, Wu L, Zhang P, Xu Y, Chen H, Li B, Tong X
Gastroenterology 2021 Aug;161(2):575-591.e16
Gastroenterology 2021 Aug;161(2):575-591.e16
Th2 Biased Immunity With Altered B Cell Profiles in Circulation of Patients With Sporotrichosis Caused by Sporothrix globosa.
Zu J, Yao L, Song Y, Cui Y, Guan M, Chen R, Zhen Y, Li S
Frontiers in immunology 2020;11:570888
Frontiers in immunology 2020;11:570888
Th17 reprogramming of T cells in systemic juvenile idiopathic arthritis.
Henderson LA, Hoyt KJ, Lee PY, Rao DA, Jonsson AH, Nguyen JP, Rutherford K, Julé AM, Charbonnier LM, Case S, Chang MH, Cohen EM, Dedeoglu F, Fuhlbrigge RC, Halyabar O, Hazen MM, Janssen E, Kim S, Lo J, Lo MS, Meidan E, Son MBF, Sundel RP, Stoll ML, Nusbaum C, Lederer JA, Chatila TA, Nigrovic PA
JCI insight 2020 Mar 26;5(6)
JCI insight 2020 Mar 26;5(6)
Human Fc Receptor-like 3 Inhibits Regulatory T Cell Function and Binds Secretory IgA.
Agarwal S, Kraus Z, Dement-Brown J, Alabi O, Starost K, Tolnay M
Cell reports 2020 Feb 4;30(5):1292-1299.e3
Cell reports 2020 Feb 4;30(5):1292-1299.e3
Epigenetically modulated FOXM1 suppresses dendritic cell maturation in pancreatic cancer and colon cancer.
Zhou Z, Chen H, Xie R, Wang H, Li S, Xu Q, Xu N, Cheng Q, Qian Y, Huang R, Shao Z, Xiang M
Molecular oncology 2019 Apr;13(4):873-893
Molecular oncology 2019 Apr;13(4):873-893
Disruption of PD-1 Enhanced the Anti-tumor Activity of Chimeric Antigen Receptor T Cells Against Hepatocellular Carcinoma.
Guo X, Jiang H, Shi B, Zhou M, Zhang H, Shi Z, Du G, Luo H, Wu X, Wang Y, Sun R, Li Z
Frontiers in pharmacology 2018;9:1118
Frontiers in pharmacology 2018;9:1118
Functionalization-dependent effects of cellulose nanofibrils on tolerogenic mechanisms of human dendritic cells.
Tomić S, Ilić N, Kokol V, Gruden-Movsesijan A, Mihajlović D, Bekić M, Sofronić-Milosavljević L, Čolić M, Vučević D
International journal of nanomedicine 2018;13:6941-6960
International journal of nanomedicine 2018;13:6941-6960
The Effects of High Mobility Group Box-1 Protein on Peripheral Treg/Th17 Balance in Patients with Atherosclerosis.
Ding JW, Zhou T, Zheng XX, Wang XA, Tong XH, Luo CY, Zhang ZQ, Yu B
Acta Cardiologica Sinica 2018 Sep;34(5):399-408
Acta Cardiologica Sinica 2018 Sep;34(5):399-408
MicroRNA-31 Targets Thymic Stromal Lymphopoietin in Mucosal Infiltrated CD4+ T Cells: A Role in Achieving Mucosal Healing in Ulcerative Colitis?
Whiteoak SR, Claridge A, Balendran CA, Harris RJ, Gwiggner M, Bondanese VP, Erlandsson F, Hansen MB, Cummings JRF, Sanchez-Elsner T
Inflammatory bowel diseases 2018 Oct 12;24(11):2377-2385
Inflammatory bowel diseases 2018 Oct 12;24(11):2377-2385
MicroRNA-210 overexpression promotes psoriasis-like inflammation by inducing Th1 and Th17 cell differentiation.
Wu R, Zeng J, Yuan J, Deng X, Huang Y, Chen L, Zhang P, Feng H, Liu Z, Wang Z, Gao X, Wu H, Wang H, Su Y, Zhao M, Lu Q
The Journal of clinical investigation 2018 Jun 1;128(6):2551-2568
The Journal of clinical investigation 2018 Jun 1;128(6):2551-2568
CRISPR-Cas9-mediated disruption of PD-1 on human T cells for adoptive cellular therapies of EBV positive gastric cancer.
Su S, Zou Z, Chen F, Ding N, Du J, Shao J, Li L, Fu Y, Hu B, Yang Y, Sha H, Meng F, Wei J, Huang X, Liu B
Oncoimmunology 2017;6(1):e1249558
Oncoimmunology 2017;6(1):e1249558
Landscape of Infiltrating T Cells in Liver Cancer Revealed by Single-Cell Sequencing.
Zheng C, Zheng L, Yoo JK, Guo H, Zhang Y, Guo X, Kang B, Hu R, Huang JY, Zhang Q, Liu Z, Dong M, Hu X, Ouyang W, Peng J, Zhang Z
Cell 2017 Jun 15;169(7):1342-1356.e16
Cell 2017 Jun 15;169(7):1342-1356.e16
Combinations of isoform-targeted histone deacetylase inhibitors and bryostatin analogues display remarkable potency to activate latent HIV without global T-cell activation.
Albert BJ, Niu A, Ramani R, Marshall GR, Wender PA, Williams RM, Ratner L, Barnes AB, Kyei GB
Scientific reports 2017 Aug 7;7(1):7456
Scientific reports 2017 Aug 7;7(1):7456
Chemical proteomic map of dimethyl fumarate-sensitive cysteines in primary human T cells.
Blewett MM, Xie J, Zaro BW, Backus KM, Altman A, Teijaro JR, Cravatt BF
Science signaling 2016 Sep 13;9(445):rs10
Science signaling 2016 Sep 13;9(445):rs10
Equilibrium of Treg/Th17 cells of peripheral blood in syphilitic patients with sero-resistance.
Zhao J, Ma J, Zhang X, Li Q, Yang X
Experimental and therapeutic medicine 2016 Jun;11(6):2300-2304
Experimental and therapeutic medicine 2016 Jun;11(6):2300-2304
Regulatory T Cell Modulation by CBP/EP300 Bromodomain Inhibition.
Ghosh S, Taylor A, Chin M, Huang HR, Conery AR, Mertz JA, Salmeron A, Dakle PJ, Mele D, Cote A, Jayaram H, Setser JW, Poy F, Hatzivassiliou G, DeAlmeida-Nagata D, Sandy P, Hatton C, Romero FA, Chiang E, Reimer T, Crawford T, Pardo E, Watson VG, Tsui V, Cochran AG, Zawadzke L, Harmange JC, Audia JE, Bryant BM, Cummings RT, Magnuson SR, Grogan JL, Bellon SF, Albrecht BK, Sims RJ 3rd, Lora JM
The Journal of biological chemistry 2016 Jun 17;291(25):13014-27
The Journal of biological chemistry 2016 Jun 17;291(25):13014-27
CRISPR-Cas9 mediated efficient PD-1 disruption on human primary T cells from cancer patients.
Su S, Hu B, Shao J, Shen B, Du J, Du Y, Zhou J, Yu L, Zhang L, Chen F, Sha H, Cheng L, Meng F, Zou Z, Huang X, Liu B
Scientific reports 2016 Jan 28;6:20070
Scientific reports 2016 Jan 28;6:20070
Human Head and Neck Squamous Cell Carcinoma-Associated Semaphorin 4D Induces Expansion of Myeloid-Derived Suppressor Cells.
Younis RH, Han KL, Webb TJ
Journal of immunology (Baltimore, Md. : 1950) 2016 Feb 1;196(3):1419-29
Journal of immunology (Baltimore, Md. : 1950) 2016 Feb 1;196(3):1419-29
Alloantigen-specific regulatory T cells generated with a chimeric antigen receptor.
MacDonald KG, Hoeppli RE, Huang Q, Gillies J, Luciani DS, Orban PC, Broady R, Levings MK
The Journal of clinical investigation 2016 Apr 1;126(4):1413-24
The Journal of clinical investigation 2016 Apr 1;126(4):1413-24
Severe impairment of regulatory T-cells and Th1-lymphocyte polarization in patients with Gaucher disease.
Sotiropoulos C, Theodorou G, Repa C, Marinakis T, Verigou E, Solomou E, Karakantza M, Symeonidis A
JIMD reports 2015;18:107-15
JIMD reports 2015;18:107-15
Age-Associated Differences in MiRNA Signatures Are Restricted to CD45RO Negative T Cells and Are Associated with Changes in the Cellular Composition, Activation and Cellular Ageing.
Teteloshvili N, Kluiver J, van der Geest KS, van der Lei RJ, Jellema P, Pawelec G, Brouwer E, Kroesen BJ, Boots AM, van den Berg A
PloS one 2015;10(9):e0137556
PloS one 2015;10(9):e0137556
Impact of Pre-Transplant Anti-T Cell Globulin (ATG) on Immune Recovery after Myeloablative Allogeneic Peripheral Blood Stem Cell Transplantation.
Servais S, Menten-Dedoyart C, Beguin Y, Seidel L, Gothot A, Daulne C, Willems E, Delens L, Humblet-Baron S, Hannon M, Baron F
PloS one 2015;10(6):e0130026
PloS one 2015;10(6):e0130026
Regulatory Dendritic Cells Restrain NK Cell IFN-γ Production through Mechanisms Involving NKp46, IL-10, and MHC Class I-Specific Inhibitory Receptors.
Spallanzani RG, Torres NI, Avila DE, Ziblat A, Iraolagoitia XL, Rossi LE, Domaica CI, Fuertes MB, Rabinovich GA, Zwirner NW
Journal of immunology (Baltimore, Md. : 1950) 2015 Sep 1;195(5):2141-8
Journal of immunology (Baltimore, Md. : 1950) 2015 Sep 1;195(5):2141-8
Fine specificities of natural regulatory T cells after IVIG therapy in patients with Kawasaki disease.
Burns JC, Touma R, Song Y, Padilla RL, Tremoulet AH, Sidney J, Sette A, Franco A
Autoimmunity 2015 May;48(3):181-8
Autoimmunity 2015 May;48(3):181-8
Safety and immunologic correlates of Melanoma GVAX, a GM-CSF secreting allogeneic melanoma cell vaccine administered in the adjuvant setting.
Lipson EJ, Sharfman WH, Chen S, McMiller TL, Pritchard TS, Salas JT, Sartorius-Mergenthaler S, Freed I, Ravi S, Wang H, Luber B, Sproul JD, Taube JM, Pardoll DM, Topalian SL
Journal of translational medicine 2015 Jul 5;13:214
Journal of translational medicine 2015 Jul 5;13:214
GITR engagement in combination with CTLA-4 blockade completely abrogates immunosuppression mediated by human liver tumor-derived regulatory T cells ex vivo.
Pedroza-Gonzalez A, Zhou G, Singh SP, Boor PP, Pan Q, Grunhagen D, de Jonge J, Tran TK, Verhoef C, IJzermans JN, Janssen H, Biermann K, Kwekkeboom J, Sprengers D
Oncoimmunology 2015 Dec;4(12):e1051297
Oncoimmunology 2015 Dec;4(12):e1051297
An increased abundance of tumor-infiltrating regulatory T cells is correlated with the progression and prognosis of pancreatic ductal adenocarcinoma.
Tang Y, Xu X, Guo S, Zhang C, Tang Y, Tian Y, Ni B, Lu B, Wang H
PloS one 2014;9(3):e91551
PloS one 2014;9(3):e91551
Immune responses induced by T-cell vaccination in patients with rheumatoid arthritis.
Ivanova I, Seledtsova G, Mamaev S, Shishkov A, Seledtsov V
Human vaccines & immunotherapeutics 2014;10(5):1221-7
Human vaccines & immunotherapeutics 2014;10(5):1221-7
Equisetum arvense (common horsetail) modulates the function of inflammatory immunocompetent cells.
Gründemann C, Lengen K, Sauer B, Garcia-Käufer M, Zehl M, Huber R
BMC complementary and alternative medicine 2014 Aug 4;14:283
BMC complementary and alternative medicine 2014 Aug 4;14:283
Neuropilin-1 expression characterizes T follicular helper (Tfh) cells activated during B cell differentiation in human secondary lymphoid organs.
Renand A, Milpied P, Rossignol J, Bruneau J, Lemonnier F, Dussiot M, Coulon S, Hermine O
PloS one 2013;8(12):e85589
PloS one 2013;8(12):e85589
Small molecule inhibitor of antigen binding and presentation by HLA-DR2b as a therapeutic strategy for the treatment of multiple sclerosis.
Ji N, Somanaboeina A, Dixit A, Kawamura K, Hayward NJ, Self C, Olson GL, Forsthuber TG
Journal of immunology (Baltimore, Md. : 1950) 2013 Nov 15;191(10):5074-84
Journal of immunology (Baltimore, Md. : 1950) 2013 Nov 15;191(10):5074-84
Calcineurin inhibitor treatment of intravenous immunoglobulin-resistant Kawasaki disease.
Tremoulet AH, Pancoast P, Franco A, Bujold M, Shimizu C, Onouchi Y, Tamamoto A, Erdem G, Dodd D, Burns JC
The Journal of pediatrics 2012 Sep;161(3):506-512.e1
The Journal of pediatrics 2012 Sep;161(3):506-512.e1
Inhibition of SLPI ameliorates disease activity in experimental autoimmune encephalomyelitis.
Müller AM, Jun E, Conlon H, Sadiq SA
BMC neuroscience 2012 Mar 21;13:30
BMC neuroscience 2012 Mar 21;13:30
Histone deacetylase inhibitors impair NK cell viability and effector functions through inhibition of activation and receptor expression.
Rossi LE, Avila DE, Spallanzani RG, Ziblat A, Fuertes MB, Lapyckyj L, Croci DO, Rabinovich GA, Domaica CI, Zwirner NW
Journal of leukocyte biology 2012 Feb;91(2):321-31
Journal of leukocyte biology 2012 Feb;91(2):321-31
Immunophenotyping at the time of diagnosis distinguishes two groups of nasopharyngeal carcinoma patients: implications for adoptive immunotherapy.
Li J, Chen QY, Mo H, Zhang YL, Huang ZF, Zeng YX
International journal of biological sciences 2011;7(5):607-17
International journal of biological sciences 2011;7(5):607-17
Immunophenotyping at the time of diagnosis distinguishes two groups of nasopharyngeal carcinoma patients: implications for adoptive immunotherapy.
Li J, Chen QY, Mo H, Zhang YL, Huang ZF, Zeng YX
International journal of biological sciences 2011;7(5):607-17
International journal of biological sciences 2011;7(5):607-17
Angiopoietin 2 stimulates TIE2-expressing monocytes to suppress T cell activation and to promote regulatory T cell expansion.
Coffelt SB, Chen YY, Muthana M, Welford AF, Tal AO, Scholz A, Plate KH, Reiss Y, Murdoch C, De Palma M, Lewis CE
Journal of immunology (Baltimore, Md. : 1950) 2011 Apr 1;186(7):4183-90
Journal of immunology (Baltimore, Md. : 1950) 2011 Apr 1;186(7):4183-90
Flt3-L increases CD4+CD25+Foxp3+ICOS+ cells in the lungs of cockroach-sensitized and -challenged mice.
McGee HS, Edwan JH, Agrawal DK
American journal of respiratory cell and molecular biology 2010 Mar;42(3):331-40
American journal of respiratory cell and molecular biology 2010 Mar;42(3):331-40
Fc receptor-like 3 protein expressed on IL-2 nonresponsive subset of human regulatory T cells.
Nagata S, Ise T, Pastan I
Journal of immunology (Baltimore, Md. : 1950) 2009 Jun 15;182(12):7518-26
Journal of immunology (Baltimore, Md. : 1950) 2009 Jun 15;182(12):7518-26
T-cell responses to survivin in cancer patients undergoing radiation therapy.
Schaue D, Comin-Anduix B, Ribas A, Zhang L, Goodglick L, Sayre JW, Debucquoy A, Haustermans K, McBride WH
Clinical cancer research : an official journal of the American Association for Cancer Research 2008 Aug 1;14(15):4883-90
Clinical cancer research : an official journal of the American Association for Cancer Research 2008 Aug 1;14(15):4883-90
T regulatory cell levels decrease in people infected with Schistosoma mansoni on effective treatment.
Watanabe K, Mwinzi PN, Black CL, Muok EM, Karanja DM, Secor WE, Colley DG
The American journal of tropical medicine and hygiene 2007 Oct;77(4):676-82
The American journal of tropical medicine and hygiene 2007 Oct;77(4):676-82
Functional inactivation of EBV-specific T-lymphocytes in nasopharyngeal carcinoma: implications for tumor immunotherapy.
Li J, Zeng XH, Mo HY, Rolén U, Gao YF, Zhang XS, Chen QY, Zhang L, Zeng MS, Li MZ, Huang WL, Wang XN, Zeng YX, Masucci MG
PloS one 2007 Nov 7;2(11):e1122
PloS one 2007 Nov 7;2(11):e1122
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Staining of PHA-stimulated normal human peripheral blood cells with Mouse IgG1 kappa Isotype Control PE (Product # 12-4714-81) (open histogram) and Anti-Human CD25 PE (filled histogram).Total viable cells were used for analysis.
- Conjugate
- Yellow dye
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Staining of human peripheral blood mononuclear cells with CD45 Pacific Blue, CD3 FITC, CD4 APC and CD25 PE. As expected based on known relative expression patterns, CD25 clone BC96 stains a subset of lymphocytes (pink), but not monocytes (orange) and granulocytes (blue).
- Conjugate
- Yellow dye
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Normal human peripheral blood cells were stained with CD4 Monoclonal Antibody, FITC (Product # 11-0047-42) and Mouse IgG1 kappa Isotype Control, PE (Product # 12-4714-82) (left) or CD25 Monoclonal Antibody, PE (right). Cells in the lymphocyte gate were used for analysis.
- Conjugate
- Yellow dye
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Conjugate
- Yellow dye
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Conjugate
- Yellow dye
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Conjugate
- Yellow dye
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 1. Flow cytometric mapping of the CD4 + CD25 + Foxp3 + cells in peripheral blood withdrawn from the syphilitic patients with sero-resistance. Foxp3, forkhead box P3.
- Conjugate
- Yellow dye
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 7 Synergistic combinations avoid global T-cell activation and induction of cytokine release. Resting CD4+ CD25- T-cells were isolated from a HIV-1 negative donor blood. Cells were incubated 24 h with 1 nM bryologs and 100 nM HDAC inhibitor. Cells were stained with fluorescent-conjugated antibodies (a) CD25-APC and (b) CD69-FITC and analyzed by FACS (see Methods). Data indicate mean values and error bars indicate mean +- s.e.m. (n >= 3). ***p < 0.001, (ANOVA). (c) ELISA results for cytokine release from CD4+ CD25- T-cells following 24 h incubation with LRA combinations (see Methods). ( d ) Toxicity profile of LRA combinations. Resting CD4+ T-cells were incubated with compounds at indicated concentrations and MTT toxicity assay was performed as described in Methods.
- Conjugate
- Yellow dye
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- FIGURE 5 Effects of disruption of PD-1 on cytotoxicity, cytokines production, and phenotype of GPC3-CAR T cells in vitro . (A) Cytotoxic activities of UTD and GPC3-CAR T cells with intact or deficient PD-1 against GPC3-positive (PLC/PRF/5 and SK-HEP-1/GPC3) and GPC3-negative (SK-HEP-1) HCC cells at an effector:target ratio of 1:1. (B,C) The production of IL-2 (B) and IFN-gamma (C) by the UTD and GPC3-CAR T cells with intact or deficient PD-1 cocultured with GPC3-positive (PLC/PRF/5 and SK-HEP-1/GPC3) or GPC3-negative (SK-HEP-1) HCC cells at an effector:target ratio of 1:1. (D) CD4 and CD8 subsets constitution of wild-type and PD-1-deficient GPC3-CAR T cells. The expressions of CD4 and CD8 on CAR T cells were measured by flow cytometry. (E) The expressions of early (CD69), and intermediate or late (CD25) activation markers on cell surface of wild-type and PD-1-deficient GPC3-CAR T cells. The expressions of CD25 and CD69 were determined by flow cytometry. Data shown were mean +- SD from triplicates. Bars, SD. * P < 0.05, ** P < 0.01, and *** P < 0.001 by one way ANOVA with Turkey post hoc test.
- Conjugate
- Yellow dye