Antibody data
- Antibody Data
- Antigen structure
- References [56]
- Comments [0]
- Validations
- Flow cytometry [1]
- Other assay [28]
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- Product number
- 12-0259-80 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD25 Monoclonal Antibody (BC96), PE, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The BC96 monoclonal antibody reacts with human CD25, the 55 kDa interleukin-2 receptor alpha chain (IL-2Ralpha). CD25 is expressed by early progenitors of T and B lineage as well as by activated mature T and B lymphocytes. By itself, CD25 binds IL-2 only with low affinity. However, CD25 associates with CD122 (IL-2 receptor beta chain) and CD132 (common gamma chain) to form the high affinity IL-2 receptor. CD25 plays a role in lymphocyte differentiating and activation/proliferation. Applications Reported: The BC96 antibody has been reported for use in flow cytometric analysis. Applications Tested: The BC96 antibody can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Yellow dye
- Isotype
- IgG
- Antibody clone number
- BC96
- Vial size
- 25 µg
- Concentration
- 0.2 mg/mL
- Storage
- 4° C, store in dark, DO NOT FREEZE!
Submitted references Advanced immunophenotyping: A powerful tool for immune profiling, drug screening, and a personalized treatment approach.
Embryonic vitamin D deficiency programs hematopoietic stem cells to induce type 2 diabetes.
Rotenone aggravates PD-like pathology in A53T mutant human α-synuclein transgenic mice in an age-dependent manner.
Endothelial dysfunction markers and immune response indices in cosmonauts' blood after long-duration space flights.
Identification of celastrol as a novel HIV-1 latency reversal agent by an image-based screen.
Epinephrine Production in Th17 Cells and Experimental Autoimmune Encephalitis.
Human endoglin-CD3 bispecific T cell engager antibody induces anti-tumor effect in vivo.
Notch4 signaling limits regulatory T-cell-mediated tissue repair and promotes severe lung inflammation in viral infections.
FABP4 facilitates inflammasome activation to induce the Treg/Th17 imbalance in preeclampsia via forming a positive feedback with IL-17A.
Numerical optimization of microfluidic vortex shedding for genome editing T cells with Cas9.
T cell receptor-dependent S-acylation of ZAP-70 controls activation of T cells.
Soluble fibrinogen‑like protein 2 levels are decreased in patients with ischemic heart failure and associated with cardiac function.
Imbalance between T helper 1 and regulatory T cells plays a detrimental role in experimental Parkinson's disease in mice.
Th2 Biased Immunity With Altered B Cell Profiles in Circulation of Patients With Sporotrichosis Caused by Sporothrix globosa.
Potential of neutrophil to lymphocyte ratio in predicting sustained remission in rheumatoid arthritis compared to other immune activation markers.
Tumors establish resistance to immunotherapy by regulating T(reg) recruitment via CCR4.
Th17 reprogramming of T cells in systemic juvenile idiopathic arthritis.
Involvement of natural killer cells in the pathogenesis of endometriosis in patients with pelvic pain.
Loading of Primary Human T Lymphocytes with Citrate-Coated Superparamagnetic Iron Oxide Nanoparticles Does Not Impair Their Activation after Polyclonal Stimulation.
Functionally specialized human CD4(+) T-cell subsets express physicochemically distinct TCRs.
T-Lymphocyte Subset Distribution and Activity in Patients With Glaucoma.
Transcriptional down-regulation of ccr5 in a subset of HIV+ controllers and their family members.
Disruption of PD-1 Enhanced the Anti-tumor Activity of Chimeric Antigen Receptor T Cells Against Hepatocellular Carcinoma.
The Effects of High Mobility Group Box-1 Protein on Peripheral Treg/Th17 Balance in Patients with Atherosclerosis.
A probiotic modulates the microbiome and immunity in multiple sclerosis.
Hormonal vitamin D up-regulates tissue-specific PD-L1 and PD-L2 surface glycoprotein expression in humans but not mice.
Alteration of Th17 and Foxp3(+) regulatory T cells in patients with unexplained recurrent spontaneous abortion before and after the therapy of hCG combined with immunoglobulin.
Combinations of isoform-targeted histone deacetylase inhibitors and bryostatin analogues display remarkable potency to activate latent HIV without global T-cell activation.
Chemical proteomic map of dimethyl fumarate-sensitive cysteines in primary human T cells.
Equilibrium of Treg/Th17 cells of peripheral blood in syphilitic patients with sero-resistance.
CRISPR-Cas9 mediated efficient PD-1 disruption on human primary T cells from cancer patients.
Human Head and Neck Squamous Cell Carcinoma-Associated Semaphorin 4D Induces Expansion of Myeloid-Derived Suppressor Cells.
Alloantigen-specific regulatory T cells generated with a chimeric antigen receptor.
Severe impairment of regulatory T-cells and Th1-lymphocyte polarization in patients with Gaucher disease.
Age-Associated Differences in MiRNA Signatures Are Restricted to CD45RO Negative T Cells and Are Associated with Changes in the Cellular Composition, Activation and Cellular Ageing.
Impact of Pre-Transplant Anti-T Cell Globulin (ATG) on Immune Recovery after Myeloablative Allogeneic Peripheral Blood Stem Cell Transplantation.
Regulatory Dendritic Cells Restrain NK Cell IFN-γ Production through Mechanisms Involving NKp46, IL-10, and MHC Class I-Specific Inhibitory Receptors.
Fine specificities of natural regulatory T cells after IVIG therapy in patients with Kawasaki disease.
Safety and immunologic correlates of Melanoma GVAX, a GM-CSF secreting allogeneic melanoma cell vaccine administered in the adjuvant setting.
GITR engagement in combination with CTLA-4 blockade completely abrogates immunosuppression mediated by human liver tumor-derived regulatory T cells ex vivo.
An increased abundance of tumor-infiltrating regulatory T cells is correlated with the progression and prognosis of pancreatic ductal adenocarcinoma.
Immune responses induced by T-cell vaccination in patients with rheumatoid arthritis.
Equisetum arvense (common horsetail) modulates the function of inflammatory immunocompetent cells.
Neuropilin-1 expression characterizes T follicular helper (Tfh) cells activated during B cell differentiation in human secondary lymphoid organs.
Small molecule inhibitor of antigen binding and presentation by HLA-DR2b as a therapeutic strategy for the treatment of multiple sclerosis.
Calcineurin inhibitor treatment of intravenous immunoglobulin-resistant Kawasaki disease.
Inhibition of SLPI ameliorates disease activity in experimental autoimmune encephalomyelitis.
Histone deacetylase inhibitors impair NK cell viability and effector functions through inhibition of activation and receptor expression.
Immunophenotyping at the time of diagnosis distinguishes two groups of nasopharyngeal carcinoma patients: implications for adoptive immunotherapy.
Immunophenotyping at the time of diagnosis distinguishes two groups of nasopharyngeal carcinoma patients: implications for adoptive immunotherapy.
Angiopoietin 2 stimulates TIE2-expressing monocytes to suppress T cell activation and to promote regulatory T cell expansion.
Flt3-L increases CD4+CD25+Foxp3+ICOS+ cells in the lungs of cockroach-sensitized and -challenged mice.
Fc receptor-like 3 protein expressed on IL-2 nonresponsive subset of human regulatory T cells.
T-cell responses to survivin in cancer patients undergoing radiation therapy.
T regulatory cell levels decrease in people infected with Schistosoma mansoni on effective treatment.
Functional inactivation of EBV-specific T-lymphocytes in nasopharyngeal carcinoma: implications for tumor immunotherapy.
Preglej T, Brinkmann M, Steiner G, Aletaha D, Göschl L, Bonelli M
Frontiers in immunology 2023;14:1096096
Frontiers in immunology 2023;14:1096096
Embryonic vitamin D deficiency programs hematopoietic stem cells to induce type 2 diabetes.
Oh J, Riek AE, Bauerle KT, Dusso A, McNerney KP, Barve RA, Darwech I, Sprague JE, Moynihan C, Zhang RM, Kutz G, Wang T, Xing X, Li D, Mrad M, Wigge NM, Castelblanco E, Collin A, Bambouskova M, Head RD, Sands MS, Bernal-Mizrachi C
Nature communications 2023 Jun 13;14(1):3278
Nature communications 2023 Jun 13;14(1):3278
Rotenone aggravates PD-like pathology in A53T mutant human α-synuclein transgenic mice in an age-dependent manner.
Chen AD, Cao JX, Chen HC, Du HL, Xi XX, Sun J, Yin J, Jing YH, Gao LP
Frontiers in aging neuroscience 2022;14:842380
Frontiers in aging neuroscience 2022;14:842380
Endothelial dysfunction markers and immune response indices in cosmonauts' blood after long-duration space flights.
Kuzichkin DS, Nichiporuk IA, Zhuravleva OA, Markin AA, Rykova MP, Zhuravleva TV, Sadova AA, Kutko OV, Shmarov VA, Ponomarev SA
NPJ microgravity 2022 Nov 2;8(1):46
NPJ microgravity 2022 Nov 2;8(1):46
Identification of celastrol as a novel HIV-1 latency reversal agent by an image-based screen.
Liu H, Hu PW, Dubrulle J, Stossi F, Nikolai BC, Mancini MA, Rice AP
PloS one 2021;16(4):e0244771
PloS one 2021;16(4):e0244771
Epinephrine Production in Th17 Cells and Experimental Autoimmune Encephalitis.
Yang P, Tian H, Zou YR, Chambon P, Ichinose H, Honig G, Diamond B, Kim SJ
Frontiers in immunology 2021;12:616583
Frontiers in immunology 2021;12:616583
Human endoglin-CD3 bispecific T cell engager antibody induces anti-tumor effect in vivo.
Zhong L, Shi W, Gan L, Liu X, Huo Y, Wu P, Zhang Z, Wu T, Peng H, Huang Y, Zhao Y, Yuan Y, Deng Z, Tang H
Theranostics 2021;11(13):6393-6406
Theranostics 2021;11(13):6393-6406
Notch4 signaling limits regulatory T-cell-mediated tissue repair and promotes severe lung inflammation in viral infections.
Harb H, Benamar M, Lai PS, Contini P, Griffith JW, Crestani E, Schmitz-Abe K, Chen Q, Fong J, Marri L, Filaci G, Del Zotto G, Pishesha N, Kolifrath S, Broggi A, Ghosh S, Gelmez MY, Oktelik FB, Cetin EA, Kiykim A, Kose M, Wang Z, Cui Y, Yu XG, Li JZ, Berra L, Stephen-Victor E, Charbonnier LM, Zanoni I, Ploegh H, Deniz G, De Palma R, Chatila TA
Immunity 2021 Jun 8;54(6):1186-1199.e7
Immunity 2021 Jun 8;54(6):1186-1199.e7
FABP4 facilitates inflammasome activation to induce the Treg/Th17 imbalance in preeclampsia via forming a positive feedback with IL-17A.
Chang GP, Yang XL, Liu W, Lin S, Yang SL, Zhao MY
Molecular therapy. Nucleic acids 2021 Jun 4;24:743-754
Molecular therapy. Nucleic acids 2021 Jun 4;24:743-754
Numerical optimization of microfluidic vortex shedding for genome editing T cells with Cas9.
Jarrell JA, Sytsma BJ, Wilson LH, Pan FL, Lau KHWJ, Kirby GTS, Lievano AA, Pawell RS
Scientific reports 2021 Jun 3;11(1):11818
Scientific reports 2021 Jun 3;11(1):11818
T cell receptor-dependent S-acylation of ZAP-70 controls activation of T cells.
Tewari R, Shayahati B, Fan Y, Akimzhanov AM
The Journal of biological chemistry 2021 Jan-Jun;296:100311
The Journal of biological chemistry 2021 Jan-Jun;296:100311
Soluble fibrinogen‑like protein 2 levels are decreased in patients with ischemic heart failure and associated with cardiac function.
You Y, Huang S, Liu H, Fan C, Liu K, Wang Z
Molecular medicine reports 2021 Aug;24(2)
Molecular medicine reports 2021 Aug;24(2)
Imbalance between T helper 1 and regulatory T cells plays a detrimental role in experimental Parkinson's disease in mice.
Li W, Luo Y, Xu H, Ma Q, Yao Q
The Journal of international medical research 2021 Apr;49(4):300060521998471
The Journal of international medical research 2021 Apr;49(4):300060521998471
Th2 Biased Immunity With Altered B Cell Profiles in Circulation of Patients With Sporotrichosis Caused by Sporothrix globosa.
Zu J, Yao L, Song Y, Cui Y, Guan M, Chen R, Zhen Y, Li S
Frontiers in immunology 2020;11:570888
Frontiers in immunology 2020;11:570888
Potential of neutrophil to lymphocyte ratio in predicting sustained remission in rheumatoid arthritis compared to other immune activation markers.
Chandrashekara S, Lingaraju DC, Renuka P, Anupama KR
The Indian journal of medical research 2020 Sep;152(3):234-243
The Indian journal of medical research 2020 Sep;152(3):234-243
Tumors establish resistance to immunotherapy by regulating T(reg) recruitment via CCR4.
Marshall LA, Marubayashi S, Jorapur A, Jacobson S, Zibinsky M, Robles O, Hu DX, Jackson JJ, Pookot D, Sanchez J, Brovarney M, Wadsworth A, Chian D, Wustrow D, Kassner PD, Cutler G, Wong B, Brockstedt DG, Talay O
Journal for immunotherapy of cancer 2020 Nov;8(2)
Journal for immunotherapy of cancer 2020 Nov;8(2)
Th17 reprogramming of T cells in systemic juvenile idiopathic arthritis.
Henderson LA, Hoyt KJ, Lee PY, Rao DA, Jonsson AH, Nguyen JP, Rutherford K, Julé AM, Charbonnier LM, Case S, Chang MH, Cohen EM, Dedeoglu F, Fuhlbrigge RC, Halyabar O, Hazen MM, Janssen E, Kim S, Lo J, Lo MS, Meidan E, Son MBF, Sundel RP, Stoll ML, Nusbaum C, Lederer JA, Chatila TA, Nigrovic PA
JCI insight 2020 Mar 26;5(6)
JCI insight 2020 Mar 26;5(6)
Involvement of natural killer cells in the pathogenesis of endometriosis in patients with pelvic pain.
He J, Xu Y, Yi M, Gu C, Zhu Y, Hu G
The Journal of international medical research 2020 Jul;48(7):300060519871407
The Journal of international medical research 2020 Jul;48(7):300060519871407
Loading of Primary Human T Lymphocytes with Citrate-Coated Superparamagnetic Iron Oxide Nanoparticles Does Not Impair Their Activation after Polyclonal Stimulation.
Mühlberger M, Unterweger H, Band J, Lehmann C, Heger L, Dudziak D, Alexiou C, Lee G, Janko C
Cells 2020 Feb 1;9(2)
Cells 2020 Feb 1;9(2)
Functionally specialized human CD4(+) T-cell subsets express physicochemically distinct TCRs.
Kasatskaya SA, Ladell K, Egorov ES, Miners KL, Davydov AN, Metsger M, Staroverov DB, Matveyshina EK, Shagina IA, Mamedov IZ, Izraelson M, Shelyakin PV, Britanova OV, Price DA, Chudakov DM
eLife 2020 Dec 8;9
eLife 2020 Dec 8;9
T-Lymphocyte Subset Distribution and Activity in Patients With Glaucoma.
Yang X, Zeng Q, Göktas E, Gopal K, Al-Aswad L, Blumberg DM, Cioffi GA, Liebmann JM, Tezel G
Investigative ophthalmology & visual science 2019 Mar 1;60(4):877-888
Investigative ophthalmology & visual science 2019 Mar 1;60(4):877-888
Transcriptional down-regulation of ccr5 in a subset of HIV+ controllers and their family members.
Gonzalo-Gil E, Rapuano PB, Ikediobi U, Leibowitz R, Mehta S, Coskun AK, Porterfield JZ, Lampkin TD, Marconi VC, Rimland D, Walker BD, Deeks S, Sutton RE
eLife 2019 Apr 9;8
eLife 2019 Apr 9;8
Disruption of PD-1 Enhanced the Anti-tumor Activity of Chimeric Antigen Receptor T Cells Against Hepatocellular Carcinoma.
Guo X, Jiang H, Shi B, Zhou M, Zhang H, Shi Z, Du G, Luo H, Wu X, Wang Y, Sun R, Li Z
Frontiers in pharmacology 2018;9:1118
Frontiers in pharmacology 2018;9:1118
The Effects of High Mobility Group Box-1 Protein on Peripheral Treg/Th17 Balance in Patients with Atherosclerosis.
Ding JW, Zhou T, Zheng XX, Wang XA, Tong XH, Luo CY, Zhang ZQ, Yu B
Acta Cardiologica Sinica 2018 Sep;34(5):399-408
Acta Cardiologica Sinica 2018 Sep;34(5):399-408
A probiotic modulates the microbiome and immunity in multiple sclerosis.
Tankou SK, Regev K, Healy BC, Tjon E, Laghi L, Cox LM, Kivisäkk P, Pierre IV, Hrishikesh L, Gandhi R, Cook S, Glanz B, Stankiewicz J, Weiner HL
Annals of neurology 2018 Jun;83(6):1147-1161
Annals of neurology 2018 Jun;83(6):1147-1161
Hormonal vitamin D up-regulates tissue-specific PD-L1 and PD-L2 surface glycoprotein expression in humans but not mice.
Dimitrov V, Bouttier M, Boukhaled G, Salehi-Tabar R, Avramescu RG, Memari B, Hasaj B, Lukacs GL, Krawczyk CM, White JH
The Journal of biological chemistry 2017 Dec 15;292(50):20657-20668
The Journal of biological chemistry 2017 Dec 15;292(50):20657-20668
Alteration of Th17 and Foxp3(+) regulatory T cells in patients with unexplained recurrent spontaneous abortion before and after the therapy of hCG combined with immunoglobulin.
Sha J, Liu F, Zhai J, Liu X, Zhang Q, Zhang B
Experimental and therapeutic medicine 2017 Aug;14(2):1114-1118
Experimental and therapeutic medicine 2017 Aug;14(2):1114-1118
Combinations of isoform-targeted histone deacetylase inhibitors and bryostatin analogues display remarkable potency to activate latent HIV without global T-cell activation.
Albert BJ, Niu A, Ramani R, Marshall GR, Wender PA, Williams RM, Ratner L, Barnes AB, Kyei GB
Scientific reports 2017 Aug 7;7(1):7456
Scientific reports 2017 Aug 7;7(1):7456
Chemical proteomic map of dimethyl fumarate-sensitive cysteines in primary human T cells.
Blewett MM, Xie J, Zaro BW, Backus KM, Altman A, Teijaro JR, Cravatt BF
Science signaling 2016 Sep 13;9(445):rs10
Science signaling 2016 Sep 13;9(445):rs10
Equilibrium of Treg/Th17 cells of peripheral blood in syphilitic patients with sero-resistance.
Zhao J, Ma J, Zhang X, Li Q, Yang X
Experimental and therapeutic medicine 2016 Jun;11(6):2300-2304
Experimental and therapeutic medicine 2016 Jun;11(6):2300-2304
CRISPR-Cas9 mediated efficient PD-1 disruption on human primary T cells from cancer patients.
Su S, Hu B, Shao J, Shen B, Du J, Du Y, Zhou J, Yu L, Zhang L, Chen F, Sha H, Cheng L, Meng F, Zou Z, Huang X, Liu B
Scientific reports 2016 Jan 28;6:20070
Scientific reports 2016 Jan 28;6:20070
Human Head and Neck Squamous Cell Carcinoma-Associated Semaphorin 4D Induces Expansion of Myeloid-Derived Suppressor Cells.
Younis RH, Han KL, Webb TJ
Journal of immunology (Baltimore, Md. : 1950) 2016 Feb 1;196(3):1419-29
Journal of immunology (Baltimore, Md. : 1950) 2016 Feb 1;196(3):1419-29
Alloantigen-specific regulatory T cells generated with a chimeric antigen receptor.
MacDonald KG, Hoeppli RE, Huang Q, Gillies J, Luciani DS, Orban PC, Broady R, Levings MK
The Journal of clinical investigation 2016 Apr 1;126(4):1413-24
The Journal of clinical investigation 2016 Apr 1;126(4):1413-24
Severe impairment of regulatory T-cells and Th1-lymphocyte polarization in patients with Gaucher disease.
Sotiropoulos C, Theodorou G, Repa C, Marinakis T, Verigou E, Solomou E, Karakantza M, Symeonidis A
JIMD reports 2015;18:107-15
JIMD reports 2015;18:107-15
Age-Associated Differences in MiRNA Signatures Are Restricted to CD45RO Negative T Cells and Are Associated with Changes in the Cellular Composition, Activation and Cellular Ageing.
Teteloshvili N, Kluiver J, van der Geest KS, van der Lei RJ, Jellema P, Pawelec G, Brouwer E, Kroesen BJ, Boots AM, van den Berg A
PloS one 2015;10(9):e0137556
PloS one 2015;10(9):e0137556
Impact of Pre-Transplant Anti-T Cell Globulin (ATG) on Immune Recovery after Myeloablative Allogeneic Peripheral Blood Stem Cell Transplantation.
Servais S, Menten-Dedoyart C, Beguin Y, Seidel L, Gothot A, Daulne C, Willems E, Delens L, Humblet-Baron S, Hannon M, Baron F
PloS one 2015;10(6):e0130026
PloS one 2015;10(6):e0130026
Regulatory Dendritic Cells Restrain NK Cell IFN-γ Production through Mechanisms Involving NKp46, IL-10, and MHC Class I-Specific Inhibitory Receptors.
Spallanzani RG, Torres NI, Avila DE, Ziblat A, Iraolagoitia XL, Rossi LE, Domaica CI, Fuertes MB, Rabinovich GA, Zwirner NW
Journal of immunology (Baltimore, Md. : 1950) 2015 Sep 1;195(5):2141-8
Journal of immunology (Baltimore, Md. : 1950) 2015 Sep 1;195(5):2141-8
Fine specificities of natural regulatory T cells after IVIG therapy in patients with Kawasaki disease.
Burns JC, Touma R, Song Y, Padilla RL, Tremoulet AH, Sidney J, Sette A, Franco A
Autoimmunity 2015 May;48(3):181-8
Autoimmunity 2015 May;48(3):181-8
Safety and immunologic correlates of Melanoma GVAX, a GM-CSF secreting allogeneic melanoma cell vaccine administered in the adjuvant setting.
Lipson EJ, Sharfman WH, Chen S, McMiller TL, Pritchard TS, Salas JT, Sartorius-Mergenthaler S, Freed I, Ravi S, Wang H, Luber B, Sproul JD, Taube JM, Pardoll DM, Topalian SL
Journal of translational medicine 2015 Jul 5;13:214
Journal of translational medicine 2015 Jul 5;13:214
GITR engagement in combination with CTLA-4 blockade completely abrogates immunosuppression mediated by human liver tumor-derived regulatory T cells ex vivo.
Pedroza-Gonzalez A, Zhou G, Singh SP, Boor PP, Pan Q, Grunhagen D, de Jonge J, Tran TK, Verhoef C, IJzermans JN, Janssen H, Biermann K, Kwekkeboom J, Sprengers D
Oncoimmunology 2015 Dec;4(12):e1051297
Oncoimmunology 2015 Dec;4(12):e1051297
An increased abundance of tumor-infiltrating regulatory T cells is correlated with the progression and prognosis of pancreatic ductal adenocarcinoma.
Tang Y, Xu X, Guo S, Zhang C, Tang Y, Tian Y, Ni B, Lu B, Wang H
PloS one 2014;9(3):e91551
PloS one 2014;9(3):e91551
Immune responses induced by T-cell vaccination in patients with rheumatoid arthritis.
Ivanova I, Seledtsova G, Mamaev S, Shishkov A, Seledtsov V
Human vaccines & immunotherapeutics 2014;10(5):1221-7
Human vaccines & immunotherapeutics 2014;10(5):1221-7
Equisetum arvense (common horsetail) modulates the function of inflammatory immunocompetent cells.
Gründemann C, Lengen K, Sauer B, Garcia-Käufer M, Zehl M, Huber R
BMC complementary and alternative medicine 2014 Aug 4;14:283
BMC complementary and alternative medicine 2014 Aug 4;14:283
Neuropilin-1 expression characterizes T follicular helper (Tfh) cells activated during B cell differentiation in human secondary lymphoid organs.
Renand A, Milpied P, Rossignol J, Bruneau J, Lemonnier F, Dussiot M, Coulon S, Hermine O
PloS one 2013;8(12):e85589
PloS one 2013;8(12):e85589
Small molecule inhibitor of antigen binding and presentation by HLA-DR2b as a therapeutic strategy for the treatment of multiple sclerosis.
Ji N, Somanaboeina A, Dixit A, Kawamura K, Hayward NJ, Self C, Olson GL, Forsthuber TG
Journal of immunology (Baltimore, Md. : 1950) 2013 Nov 15;191(10):5074-84
Journal of immunology (Baltimore, Md. : 1950) 2013 Nov 15;191(10):5074-84
Calcineurin inhibitor treatment of intravenous immunoglobulin-resistant Kawasaki disease.
Tremoulet AH, Pancoast P, Franco A, Bujold M, Shimizu C, Onouchi Y, Tamamoto A, Erdem G, Dodd D, Burns JC
The Journal of pediatrics 2012 Sep;161(3):506-512.e1
The Journal of pediatrics 2012 Sep;161(3):506-512.e1
Inhibition of SLPI ameliorates disease activity in experimental autoimmune encephalomyelitis.
Müller AM, Jun E, Conlon H, Sadiq SA
BMC neuroscience 2012 Mar 21;13:30
BMC neuroscience 2012 Mar 21;13:30
Histone deacetylase inhibitors impair NK cell viability and effector functions through inhibition of activation and receptor expression.
Rossi LE, Avila DE, Spallanzani RG, Ziblat A, Fuertes MB, Lapyckyj L, Croci DO, Rabinovich GA, Domaica CI, Zwirner NW
Journal of leukocyte biology 2012 Feb;91(2):321-31
Journal of leukocyte biology 2012 Feb;91(2):321-31
Immunophenotyping at the time of diagnosis distinguishes two groups of nasopharyngeal carcinoma patients: implications for adoptive immunotherapy.
Li J, Chen QY, Mo H, Zhang YL, Huang ZF, Zeng YX
International journal of biological sciences 2011;7(5):607-17
International journal of biological sciences 2011;7(5):607-17
Immunophenotyping at the time of diagnosis distinguishes two groups of nasopharyngeal carcinoma patients: implications for adoptive immunotherapy.
Li J, Chen QY, Mo H, Zhang YL, Huang ZF, Zeng YX
International journal of biological sciences 2011;7(5):607-17
International journal of biological sciences 2011;7(5):607-17
Angiopoietin 2 stimulates TIE2-expressing monocytes to suppress T cell activation and to promote regulatory T cell expansion.
Coffelt SB, Chen YY, Muthana M, Welford AF, Tal AO, Scholz A, Plate KH, Reiss Y, Murdoch C, De Palma M, Lewis CE
Journal of immunology (Baltimore, Md. : 1950) 2011 Apr 1;186(7):4183-90
Journal of immunology (Baltimore, Md. : 1950) 2011 Apr 1;186(7):4183-90
Flt3-L increases CD4+CD25+Foxp3+ICOS+ cells in the lungs of cockroach-sensitized and -challenged mice.
McGee HS, Edwan JH, Agrawal DK
American journal of respiratory cell and molecular biology 2010 Mar;42(3):331-40
American journal of respiratory cell and molecular biology 2010 Mar;42(3):331-40
Fc receptor-like 3 protein expressed on IL-2 nonresponsive subset of human regulatory T cells.
Nagata S, Ise T, Pastan I
Journal of immunology (Baltimore, Md. : 1950) 2009 Jun 15;182(12):7518-26
Journal of immunology (Baltimore, Md. : 1950) 2009 Jun 15;182(12):7518-26
T-cell responses to survivin in cancer patients undergoing radiation therapy.
Schaue D, Comin-Anduix B, Ribas A, Zhang L, Goodglick L, Sayre JW, Debucquoy A, Haustermans K, McBride WH
Clinical cancer research : an official journal of the American Association for Cancer Research 2008 Aug 1;14(15):4883-90
Clinical cancer research : an official journal of the American Association for Cancer Research 2008 Aug 1;14(15):4883-90
T regulatory cell levels decrease in people infected with Schistosoma mansoni on effective treatment.
Watanabe K, Mwinzi PN, Black CL, Muok EM, Karanja DM, Secor WE, Colley DG
The American journal of tropical medicine and hygiene 2007 Oct;77(4):676-82
The American journal of tropical medicine and hygiene 2007 Oct;77(4):676-82
Functional inactivation of EBV-specific T-lymphocytes in nasopharyngeal carcinoma: implications for tumor immunotherapy.
Li J, Zeng XH, Mo HY, Rolén U, Gao YF, Zhang XS, Chen QY, Zhang L, Zeng MS, Li MZ, Huang WL, Wang XN, Zeng YX, Masucci MG
PloS one 2007 Nov 7;2(11):e1122
PloS one 2007 Nov 7;2(11):e1122
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Supportive validation
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- Staining of human peripheral blood mononuclear cells with CD45 Pacific Blue, CD3 FITC, CD4 APC and CD25 PE. As expected based on known relative expression patterns, CD25 clone BC96 stains a subset of lymphocytes (pink), but not monocytes (orange) and granulocytes (blue).
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- Figure 5 S-acylation of ZAP-70 is required for T cell activation. A , IL-2 production by P116 (ZAP-70 -/-) Jurkat T cells stably rescued with WT or acylation-deficient C564R ZAP-70. IL-2 concentrations were measured by ELISA in supernatants from resting cells or cells stimulated for 24 h with plate-bound anti-CD3 antibody. Data shown are representative of three independent biological repeats and represented as mean +- SEM. B and C , Expression of CD25 and CD69 T cell surface activation markers by P116 stably rescued with WT or C564R ZAP-70. Cells were stimulated for 24 h with plate-bound anti-CD3 antibody and analyzed by flow cytometry. D and E , Quantification of CD25 and CD69 expression measured by flow cytometry. Data shown are pooled from three independent biological repeats and represented as mean +- SEM.
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- Figure 1. Flow cytometric mapping of the CD4 + CD25 + Foxp3 + cells in peripheral blood withdrawn from the syphilitic patients with sero-resistance. Foxp3, forkhead box P3.
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- Figure 7 Synergistic combinations avoid global T-cell activation and induction of cytokine release. Resting CD4+ CD25- T-cells were isolated from a HIV-1 negative donor blood. Cells were incubated 24 h with 1 nM bryologs and 100 nM HDAC inhibitor. Cells were stained with fluorescent-conjugated antibodies (a) CD25-APC and (b) CD69-FITC and analyzed by FACS (see Methods). Data indicate mean values and error bars indicate mean +- s.e.m. (n >= 3). ***p < 0.001, (ANOVA). (c) ELISA results for cytokine release from CD4+ CD25- T-cells following 24 h incubation with LRA combinations (see Methods). ( d ) Toxicity profile of LRA combinations. Resting CD4+ T-cells were incubated with compounds at indicated concentrations and MTT toxicity assay was performed as described in Methods.
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- Figure 5 Notch4 deficiency reprograms the lung Treg cell transcriptome of poly(I:C)-treated mice (A-C) Volcano plot (A), heatmap (B), and pathway analysis (C) of gene transcripts of lung Treg cells isolated from Foxp3 YFPCre and Foxp3 YFPCre Notch4 Delta/Delta mice treated with poly(I:C) (n = 4 and n = 5, respectively). (D) Flow cytometry histograms and graphical representation of lung tissue Treg cell expression of CD25, Helios, CTLA4, ICOS, and OX40 in Foxp3 YFPCre and Foxp3 YFPCre Notch4 Delta/Delta mice sampled on day 7 after poly(I:C) treatment (n = 5 for each time point). (E-H) Flow cytometry analysis (E and G) and graphical representation (F and H) of Helios expression in Foxp3 + Notch4 + and Foxp3 + Notch4 - lung tissue Treg cells in Foxp3 YFPCre mice sampled at the indicated dates after poly(I:C) treatment (n = 5 for each time point). Each symbol represents one mouse. Numbers in flow plots indicate percentages. Error bars indicate SEM. Statistical tests: pairwise comparisons of differential gene expression were computed using DESeq2 (A-C); Student''s unpaired two-tailed t test (D) and two-way ANOVA with Sidak''s post hoc analysis (F and H). * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.
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- Figure 1. Patients with Parkinson's disease (PD) and mice with experimental PD exhibit decreased regulatory T (Treg) and increased T helper 1 (Th1) cell numbers in the blood. Treg cells were defined as CD3+CD4+CD25+FoxP3+ cells, whereas Th1 cells were identified as CD3+CD4+T-bet+ cells. (a) Representative plots of Treg and Th1 cells in patients with PD and healthy volunteers (HVs). (b) Representative plots of Treg and Th1 cells in control mice (Saline) and 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-treated mice. (c) Patients with PD exhibited lower Treg cell levels and greater Th1 cell levels in the blood than HVs. n = 20. ****, p < 0.0001 by a two-tailed Student's t tests. (d) MPTP-induced experimental PD mice had fewer Treg and more Th1 cells in the circulation than saline-treated mice. n = 9/group in each experiment performed in triplicate. ***, p < 0.001; ****, p < 0.0001, according to Student's t test.
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- Figure 4 Surface marker expression and cytokine secretion levels minimally perturbed with Cas9 and uVS . ( A ) CD25, and ( B ) PD-1 expression levels were quantified in cells transfected with uVS or electroporation. Surface marker expression levels were measured in the total live cell and TRAC-1 KO live population via flow cytometry at Days 1, 4, 7, 10 and 14 post-transfection. ( C ) ELISA quantification of IFNgamma supernatant levels in transfected and non-transfected cells at Day 1, 4, 7, and 10 post-transfection. Non-targeting gRNA as a Cas9-RNP complex served as a non-editing control. Cells mixed with P3 buffer (EP--non-transfection) or Opti-MEM buffer ( uVS --non-transfection) that were not transfected served as non-transfection controls for electroporation and uVS samples, respectively. Data represent mean +- SD of n = 3 samples per condition. *P < 0.05, **P < 0.01 by unpaired, two-tailed, heteroscedastic t tests. Figure generated using Adobe Illustrator Creative Cloud (adobe.com/products/illustrator.html) and GraphPad Prism 9 (graphpad.com/scientific-software/prism/).
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- Figure 1. The percentage of T helper 17 (Th17) and regulatory T (Treg) cells in peripheral blood mononuclear cells (PBMCs) and the Th17/Treg ratio varies in patients with a successful pregnancy after immunotherapy. The percentage of Th17 and Treg cells in PBMCs were detected by flow cytometry in patients with unexplained recurrent spontaneous abortion (URSA) before and after therapy. Representative CD3 + CD8 + IL-17A + flow cytometry plots from patients with URSA are shown (A) before and (B) after therapy. Representative CD4 + CD25 + Foxp3 + flow cytometry plots from patients with URSA are shown (C) before and (D) after therapy. (E) The percentage of Th17 cells in PBMCs significantly decreased after therapy. The percentage of Treg cells in PBMCs significantly increased after therapy (*P
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- Figure 1 Elevation of FABP4 is accompanied with Treg/Th17 imbalance in PE (A) The serum level of FABP4 was determined by ELISA (normal pregnant women, n = 10; PE, n = 20). (B) The immunoreactivities of FABP4, IL-17A, and FOXP3 in placental tissues were monitored by IHC analysis. (C and D) The percentages of Treg (C) and Th17 cells (D) in peripheral blood were assessed by FACS (normal pregnant women, n = 10; PE, n = 20). (E) The serum level of IL-17A was determined by ELISA (normal pregnant women, n = 10; PE, n = 20). (F) Pearson's correlation analysis between IL-17A and FABP4 expression. Data were representative images. *p < 0.05.
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- Figure 1. Serum sFGL2 levels and circulating Tregs frequencies are decreased in patients with IHF. (A) Serum sFGL2 levels were analyzed by ELISA. (B) The frequency of Tregs was compared in each group. (C) Representative FSC/SSC pseudo-color density image shows the gated CD4 + T cells and representative fluorescence-activated cell sorting plots of CD4 + CD25 + Foxp3 + Tregs from a single patient in each group. ***P
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- Figure 2. Serum sFGL2 levels and circulating Tregs frequencies are decreased with the elevation of NYHA classification. (A) sFGL2 levels were analyzed by ELISA. (B) The proportion of Tregs was compared in each subgroup. (C) Representative fluorescence-activated cell sorting plots of CD4 + CD25 + Foxp3 + Tregs from a single person in each subgroup. **P
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- Figure 3. Serum sFGL2 levels and circulating Tregs frequencies are decreased with the reduction of LVEF. (A) sFGL2 levels were analyzed by ELISA. (B) The proportion of Tregs was compared in each subgroup. (C) Representative fluorescence-activated cell sorting plots of CD4 + CD25 + Foxp3 + Tregs from a single person in each subgroup. *P
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- Figure 2 Tregs' frequency and the ratio of effector T cells/Tregs in PBMCs of patients and HC. (A) PBMCs were stained with CD4, CD25 and CD127 mAbs. CD4 + T cells were gated according to CD4 staining and SSC and the proportion of CD4 + CD25 + CD127 - Tregs in CD4 + T cells were determined. The graphs are representative for patients, indicated subgroups and HC. Mean value of Tregs' percentage is shown in each target quadrant. (B-E) Statistical graphs for comparing Tregs' percentage and Th/Tregs ratios of the whole patients and indicated subgroups with HC. (SP, n = 46; SD, n = 20; LD, n = 26, FF, n = 30; LF, n = 16; HC, n = 25). Error bars represent mean+-SD. ** P < 0.01, * P < 0.05, and NS P >= 0.05.
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- Figure 3 hEND-CD3/BiTE activates T cells, stimulates T-cell proliferation, and increases secretion of Th1 cytokines. (A) PBMCs were co-cultured with 293T (grey signal) or 293T-hE (pink signal) cells in the presence of hEND-CD3/BiTE. The expression of T-cell activation markers CD69 (left panels) and CD25 (right panels) was detected in CD4 + (upper rows) and CD8 + (lower rows) by flow cytometry. (B) Proliferation of CFSE-labeled T cells in response to hEND-CD3/BiTE and other indicated reagents in the solid phase was measured using flow cytometry. The proliferation index was calculated using the ModFit LT software. (C) Supernatants were collected from the co-cultures of PBMCs with 293T (white bar) or 293T-hE cells (purple bar) in the presence of hEND-CD3/BiTE. The secretion of Th1 cytokines, including IFN-gamma, and TNF-alpha, and Th2 cytokine IL10 was measured using ELISA assays. * P < 0.001.
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- 10.7554/eLife.44360.007 Figure 3. Lower proliferative responses and CCR2 and CCR5 cell surface levels in activated CD4 +T cells from ECr/VCrs. ( A ) Reduced CD69, but not CD25 levels in activated CD4 +T cells from ECr/VCrs. Graph shows representative data N = 13 (Ctrl), n = 9 (EC/VC) and n = 21 (ECr/VCr). ( B ) Comparable frequencies of naive CD45RA + and memory CD45RO + T cells after anti-CD3/CD28 activation between groups (n = 2 per group). ( C ) CCR5 and CCR2 cell surface levels measured by flow cytometry are reduced in freshly thawed (NS, non-stimulated) and activated CD4 +T cells (anti-CD3/28) from ECr/VCr. ( D ) Percentages of CCR5 +in effector memory (EM) and central memory (CM) compartments of activated CD4 +T cells (n = 2 per group). ( E ) Reduced CCR2 and CCR5 cell surface levels, expressed as MFI, in activated (anti-CD3/28) CD4 +T cells from ECr/VCr. Data in D-E shown pooled results from different experiments with n = 10 (Ctrl and EC/VC) and n = 19 (ECr/VCr). ( F ) Positive correlation between CCR2 and CCR5 cell surface levels. ( G ) Positive correlation observed between ccr2/ccr5 RNA levels and cell surface expression. Values obtained using the non-parametric Spearman correlation test. *p
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- 10.1371/journal.pone.0244771.g004 Fig 4 Celastrol does not activate resting CD4+ T cells. Resting CD4 + T cells were isolated from three healthy donors and treated with 400 nM celastrol, 200 nM JQ1, 400 nM vorinostat, 20 nM romidepsin, or 0.2 ng/ml bryostatin for 24 hours. Expression of CD25 and CD69 was quantified by flow cytometry.
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- Fig. 3 Flow cytometric analysis of CD4 + CD25 + FOXP3 + cells. ( A ) Lymphocytes are gated in forward light scatter (FS) vs. side light scatter (SS) dot plot, then ( B ) CD3+ cells gated in CD3- E610 vs. SS, followed by ( C ) CD4+ gating in CD3- E610 vs. CD4- FITC. ( D ) CD4+ cells gated are plotted CD25-PC5 vs. FoxP3-PE for assessing CD4+CD25+FoxP3+ (C2) cell expression.
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- FIGURE 6 The effect of rotenone on subpopulations of Peyer's patches lymphocytes in halpha-syn +/- mice. Flow cytometry was used to detect the changes in lymphocyte subsets, namely, CD4 + , CD8 + , and CD4 + CD25 + lymphocytes, respectively, in Peyer's patches in mice of different ages. (A) Changes of lymphocyte subsets in Peyer's patches of 6-month-old halpha-syn +/- mice by flow cytometry, n = 6. (B) Statistics of the number of cells in Peyer's patches of 6-month-old halpha-syn +/- mice. (C) Changes of lymphocyte subsets in Peyer's patches of 12-month-old halpha-syn +/- mice by flow cytometry, n = 6. (D) Statistics of the number of cells in Peyer's patches of 12-month-old halpha-syn +/- mice.
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- FIGURE 5 Effects of disruption of PD-1 on cytotoxicity, cytokines production, and phenotype of GPC3-CAR T cells in vitro . (A) Cytotoxic activities of UTD and GPC3-CAR T cells with intact or deficient PD-1 against GPC3-positive (PLC/PRF/5 and SK-HEP-1/GPC3) and GPC3-negative (SK-HEP-1) HCC cells at an effector:target ratio of 1:1. (B,C) The production of IL-2 (B) and IFN-gamma (C) by the UTD and GPC3-CAR T cells with intact or deficient PD-1 cocultured with GPC3-positive (PLC/PRF/5 and SK-HEP-1/GPC3) or GPC3-negative (SK-HEP-1) HCC cells at an effector:target ratio of 1:1. (D) CD4 and CD8 subsets constitution of wild-type and PD-1-deficient GPC3-CAR T cells. The expressions of CD4 and CD8 on CAR T cells were measured by flow cytometry. (E) The expressions of early (CD69), and intermediate or late (CD25) activation markers on cell surface of wild-type and PD-1-deficient GPC3-CAR T cells. The expressions of CD25 and CD69 were determined by flow cytometry. Data shown were mean +- SD from triplicates. Bars, SD. * P < 0.05, ** P < 0.01, and *** P < 0.001 by one way ANOVA with Turkey post hoc test.
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- Figure 3 T-lymphocyte subset distribution in glaucoma. Mononuclear cells isolated from peripheral blood samples from glaucoma (n = 32) and control (n = 21) groups were analyzed by multicolor flow cytometry for T-cell subset markers. (A) Similar to CD4+ Tregs, the percentage of CD8+/CD25+/FoxP3+ Tregs (CD8-Tregs) was also significantly lower in glaucomatous samples than nonglaucomatous controls (ANOVA, P < 0.001). (B) Shown are representative flow cytometry images after CD8/CD25/FoxP3 immunostaining for Tregs.
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