Antibody data
- Antibody Data
- Antigen structure
- References [15]
- Comments [0]
- Validations
- Flow cytometry [1]
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- Product number
- 15-0259-41 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-CD25 Monoclonal Antibody (BC96), PE-Cyanine5, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The BC96 monoclonal antibody reacts with human CD25, the 55 kDa interleukin-2 receptor alpha chain (IL-2Ralpha). CD25 is expressed by early progenitors of T and B lineage as well as by activated mature T and B lymphocytes. By itself, CD25 binds IL-2 only with low affinity. However, CD25 associates with CD122 (IL-2 receptor beta chain) and CD132 (common gamma chain) to form the high affinity IL-2 receptor. CD25 plays a role in lymphocyte differentiating and activation/proliferation. Applications Reported: This BC96 antibody has been reported for use in flow cytometric analysis. Applications Tested: This BC96 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 20 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically. Excitation: 488-561 nm; Emission: 667 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- BC96
- Vial size
- 25 Tests
- Concentration
- 5 µL/Test
- Storage
- 4° C, store in dark, DO NOT FREEZE!
Submitted references Disruption of PD-1 Enhanced the Anti-tumor Activity of Chimeric Antigen Receptor T Cells Against Hepatocellular Carcinoma.
Functionalization-dependent effects of cellulose nanofibrils on tolerogenic mechanisms of human dendritic cells.
Combinations of isoform-targeted histone deacetylase inhibitors and bryostatin analogues display remarkable potency to activate latent HIV without global T-cell activation.
Equilibrium of Treg/Th17 cells of peripheral blood in syphilitic patients with sero-resistance.
Function of Treg Cells Decreased in Patients With Systemic Lupus Erythematosus Due To the Effect of Prolactin.
Human Head and Neck Squamous Cell Carcinoma-Associated Semaphorin 4D Induces Expansion of Myeloid-Derived Suppressor Cells.
Alloantigen-specific regulatory T cells generated with a chimeric antigen receptor.
The disturbance of TH17-Treg cell balance in adenomyosis.
Incomplete normalization of regulatory t-cell frequency in the gut mucosa of Colombian HIV-infected patients receiving long-term antiretroviral treatment.
High CCR6/CCR7 expression and Foxp3+ Treg cell number are positively related to the progression of laryngeal squamous cell carcinoma.
HIV-induced T-cell activation/exhaustion in rectal mucosa is controlled only partially by antiretroviral treatment.
Chronically HIV-1 Infected Patients Exhibit Low Frequencies of CD25+ Regulatory T Cells.
Prolactin down-regulates CD4+CD25hiCD127low/- regulatory T cell function in humans.
CpG and non-CpG oligodeoxynucleotides directly costimulate mouse and human CD4+ T cells through a TLR9- and MyD88-independent mechanism.
Fc receptor-like 3 protein expressed on IL-2 nonresponsive subset of human regulatory T cells.
Guo X, Jiang H, Shi B, Zhou M, Zhang H, Shi Z, Du G, Luo H, Wu X, Wang Y, Sun R, Li Z
Frontiers in pharmacology 2018;9:1118
Frontiers in pharmacology 2018;9:1118
Functionalization-dependent effects of cellulose nanofibrils on tolerogenic mechanisms of human dendritic cells.
Tomić S, Ilić N, Kokol V, Gruden-Movsesijan A, Mihajlović D, Bekić M, Sofronić-Milosavljević L, Čolić M, Vučević D
International journal of nanomedicine 2018;13:6941-6960
International journal of nanomedicine 2018;13:6941-6960
Combinations of isoform-targeted histone deacetylase inhibitors and bryostatin analogues display remarkable potency to activate latent HIV without global T-cell activation.
Albert BJ, Niu A, Ramani R, Marshall GR, Wender PA, Williams RM, Ratner L, Barnes AB, Kyei GB
Scientific reports 2017 Aug 7;7(1):7456
Scientific reports 2017 Aug 7;7(1):7456
Equilibrium of Treg/Th17 cells of peripheral blood in syphilitic patients with sero-resistance.
Zhao J, Ma J, Zhang X, Li Q, Yang X
Experimental and therapeutic medicine 2016 Jun;11(6):2300-2304
Experimental and therapeutic medicine 2016 Jun;11(6):2300-2304
Function of Treg Cells Decreased in Patients With Systemic Lupus Erythematosus Due To the Effect of Prolactin.
Legorreta-Haquet MV, Chávez-Rueda K, Chávez-Sánchez L, Cervera-Castillo H, Zenteno-Galindo E, Barile-Fabris L, Burgos-Vargas R, Álvarez-Hernández E, Blanco-Favela F
Medicine 2016 Feb;95(5):e2384
Medicine 2016 Feb;95(5):e2384
Human Head and Neck Squamous Cell Carcinoma-Associated Semaphorin 4D Induces Expansion of Myeloid-Derived Suppressor Cells.
Younis RH, Han KL, Webb TJ
Journal of immunology (Baltimore, Md. : 1950) 2016 Feb 1;196(3):1419-29
Journal of immunology (Baltimore, Md. : 1950) 2016 Feb 1;196(3):1419-29
Alloantigen-specific regulatory T cells generated with a chimeric antigen receptor.
MacDonald KG, Hoeppli RE, Huang Q, Gillies J, Luciani DS, Orban PC, Broady R, Levings MK
The Journal of clinical investigation 2016 Apr 1;126(4):1413-24
The Journal of clinical investigation 2016 Apr 1;126(4):1413-24
The disturbance of TH17-Treg cell balance in adenomyosis.
Gui T, Chen C, Zhang Z, Tang W, Qian R, Ma X, Cao P, Wan G
Fertility and sterility 2014 Feb;101(2):506-14
Fertility and sterility 2014 Feb;101(2):506-14
Incomplete normalization of regulatory t-cell frequency in the gut mucosa of Colombian HIV-infected patients receiving long-term antiretroviral treatment.
Rueda CM, Velilla PA, Chougnet CA, Rugeles MT
PloS one 2013;8(8):e71062
PloS one 2013;8(8):e71062
High CCR6/CCR7 expression and Foxp3+ Treg cell number are positively related to the progression of laryngeal squamous cell carcinoma.
Chen B, Zhang D, Zhou J, Li Q, Zhou L, Li SM, Zhu L, Chou KY, Zhou L, Tao L, Lu LM
Oncology reports 2013 Sep;30(3):1380-90
Oncology reports 2013 Sep;30(3):1380-90
HIV-induced T-cell activation/exhaustion in rectal mucosa is controlled only partially by antiretroviral treatment.
Rueda CM, Velilla PA, Chougnet CA, Montoya CJ, Rugeles MT
PloS one 2012;7(1):e30307
PloS one 2012;7(1):e30307
Chronically HIV-1 Infected Patients Exhibit Low Frequencies of CD25+ Regulatory T Cells.
Rios CM, Velilla PA, Rugeles MT
The open virology journal 2012;6:49-58
The open virology journal 2012;6:49-58
Prolactin down-regulates CD4+CD25hiCD127low/- regulatory T cell function in humans.
Legorreta-Haquet MV, Chávez-Rueda K, Montoya-Díaz E, Arriaga-Pizano L, Silva-García R, Chávez-Sánchez L, Moreno-Lafont M, Zenteno-Galindo E, Blanco-Favela F
Journal of molecular endocrinology 2012 Feb;48(1):77-85
Journal of molecular endocrinology 2012 Feb;48(1):77-85
CpG and non-CpG oligodeoxynucleotides directly costimulate mouse and human CD4+ T cells through a TLR9- and MyD88-independent mechanism.
Landrigan A, Wong MT, Utz PJ
Journal of immunology (Baltimore, Md. : 1950) 2011 Sep 15;187(6):3033-43
Journal of immunology (Baltimore, Md. : 1950) 2011 Sep 15;187(6):3033-43
Fc receptor-like 3 protein expressed on IL-2 nonresponsive subset of human regulatory T cells.
Nagata S, Ise T, Pastan I
Journal of immunology (Baltimore, Md. : 1950) 2009 Jun 15;182(12):7518-26
Journal of immunology (Baltimore, Md. : 1950) 2009 Jun 15;182(12):7518-26
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Supportive validation
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- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Staining of 3-day PHA activated normal human peripheral blood mononuclear cells with Mouse IgG1 kappa Isotype Control PE-Cyanine5 (Product # 15-4714-81) (open histogram) or Anti-Human CD25 PE-Cyanine5 (filled histogram).Total cells were used for analysis.