60009

antibody from STEMCELL Technologies Inc

Targeting: IL2RA CD25, IDDM10, IL2R

Immunohistochemistry (Recommended by provider)

Flow cytometry (Recommended by provider)

Blocking/Neutralizing (Recommended by provider)

Antibody Data

Product number

60009

Provider

STEMCELL Technologies Inc

Product name

Anti-Mouse CD25 Antibody, Clone PC61.5

Antibody type

Monoclonal

Description

The PC61.5 antibody reacts with mouse CD25 (low-affinity interleukin?2 receptor ? chain or IL-2R?), which is an ~55 kDa type 1 transmembrane glycoprotein expressed on T and B cell progenitors, activated (but not resting) T and B cells, regulatory T (Treg) cells, dendritic cells, and activated monocytes and macrophages. Expression of CD25, together with CD4 and FOXP3, is considered a phenotypic signature for Treg cells. CD25 per se has low affinity for its IL-2 ligand but associates with CD122 (IL-2R?) and CD132 (IL-2R?) to form the high-affinity IL-2R receptor. CD25 is necessary for receptor clustering and induction of signaling pathways involved in the activation and proliferation of lymphocytes. Binding of the PC61.5 antibody inhibits binding of IL-2 to the low- and high-affinity receptors, which may result in inhibition of IL-2-dependent proliferation. The epitope recognized by PC61.5 is distinct from the IL-2 binding site and from the epitopes recognized by antibodies 7D4 and 3C7.

Reactivity

Mouse

Host

Mouse

Isotype

IgG

Vial size

100 µg

Storage

2 - 8°C