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Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Flow cytometry [1]
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- Product number
- 302621 - Provider product page
- Provider
- BioLegend
- Proper citation
- BioLegend Cat#302621, RRID:AB_493754
- Product name
- Alexa Fluor® 700 anti-human CD25 [BC96]
- Antibody type
- Monoclonal
- Antigen
- CD25
- Description
- CD25 is a 55 kD type I transmembrane glycoprotein also known as the low affinity IL-2 receptor α chain or Tac. It is expressed on progenitor lymphocytes, activated T and B cells, and activated monocytes/macrophages. CD25 is also expressed on a subset of non-stimulated CD4+ T cells termed T regulatory cells. CD25 associates with the IL-2 receptor β (CD122) and common γ chains (CD132) to form the high affinity IL-2R complex.
- Reactivity
- Human, Simian
- Host
- Mouse
- Conjugate
- Far-Red dye, Near infrared dye
- Isotype
- IgG
- Vial size
- 25 µg
- Concentration
- 0.5 mg/ml
- Storage
- The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Submitted references Human T cells express CD25 and Foxp3 upon activation and exhibit effector/memory phenotypes without any regulatory/suppressor function.
Kmieciak M, Gowda M, Graham L, Godder K, Bear HD, Marincola FM, Manjili MH
Journal of translational medicine 2009 Oct 22;7:89
Journal of translational medicine 2009 Oct 22;7:89
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- BioLegend (provider)
- Main image
- Experimental details
- PHA-stimulated human peripheral blood mononuclear cells (day-3) stained with BC96 Alexa Fluor® 700
- Conjugate
- Near infrared dye
