M10AR

antibody from Invitrogen Antibodies

Targeting: IL2RA CD25, IDDM10, IL2R

Western blot (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Recommended by provider)

Flow cytometry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

M10AR

Provider

Invitrogen Antibodies

Product name

Anti-CD25 Monoclonal Antibody (2R12)

Antibody type

Monoclonal

Antigen

Other

Description

M10AR targets CD25 in ICC/IF, FACS, IHC, IP, and WB applications and shows reactivity with Human samples. The M10AR immunogen is p55 alpha chain of the human IL-2 receptor. M10AR detects CD25 which has a predicted molecular weight of approximately 28 kDa.

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

2R12

Vial size

100 tests

Concentration

20 µg/ml

Storage

4° C

References

Submitted references

Heterogeneous immunophenotype of granular lymphocyte expansions: differential expression of the CD8 alpha and CD8 beta chains.

de Totero D, Tazzari PL, DiSanto JP, di Celle PF, Raspadori D, Conte R, Gobbi M, Ferrara GB, Flomenberg N, Lauria F
Blood 1992 Oct 1;80(7):1765-73

---

Structural and functional characterization of IL 2 receptors on activated human B cells.

Boyd AW, Fisher DC, Fox DA, Schlossman SF, Nadler LM
Journal of immunology (Baltimore, Md. : 1950) 1985 Apr;134(4):2387-92

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of CD25 (green) showing staining in the cytoplasm of Jurkat cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a CD25 monoclonal antibody (Product # M10AR) in 3% BSA-PBS at a dilution of 1:20 and incubated overnight at 4ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry analysis of CD25 in Jurkat cells (green) compared to an isotype control (blue). Cells were harvested, adjusted to a concentration of 1-5x10^6 cells/ml, fixed with 2% paraformaldehyde and washed with PBS. Cells were blocked with a 2% solution of BSA-PBS for 30 min at room temperature and incubated with a CD25 monoclonal antibody (Product # M10AR) at a dilution of 1:10 for 60 min at room temperature. Cells were then incubated for 40 min at room temperature in the dark using a Dylight 488-conjugated secondary antibody and re-suspended in PBS for FACS analysis.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP-MS enrichment of IL2RA (LFQ intensity): IL2RA was enriched 2108-fold from SR lysate compared to background proteins, using the optimized IP-MS workflow with Pierce MS-Compatible Magnetic IP Kit protein A/G (Product # 90409) and IL2RA antibody (Product # M10AR). The STRING database (www.string-db.org) was used to identify the protein interactor list. See more information on IP-MS verification of antibody selectivity.