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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [2]
- Flow cytometry [1]
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- Product number
- RQ6563 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- IL-2 receptor alpha Antibody / Cd25
- Antibody type
- Polyclonal
- Description
- This highly specific IL-2 receptor alpha antibody is suitable for use in Western blot/Immunohistochemistry/Flow cytometry applications with mouse and rat samples.
- Reactivity
- Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 100 ug
- Concentration
- 0.5mg/ml if reconstituted with 0.2ml sterile DI water
- Storage
- After reconstitution, the IL-2 receptor alpha antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of 1) mouse ANA-1, 2) mouse NIH 3T3, 3) mouse RAW264.7, 4) mouse HEPA-1, 5) rat C6, 6) rat NRK and 7) rat RH35 cell lysate with IL-2 receptor alpha antibody. Expected molecular weight: 25~45 kDa depending on level of glycosylation.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE mouse spleen tissue with IL-2 receptor alpha antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE rat small intestine tissue with IL-2 receptor alpha antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Flow cytometry testing of mouse HEPA1-6 cells with IL-2 receptor alpha antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= IL-2 receptor alpha antibody.
