Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunocytochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- AF9029-100 - Provider product page
- Provider
- R&D Systems
- Product name
- Rat 4-1BB/TNFRSF9/CD137 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects rat 4-1BB in direct ELISAs.
- Reactivity
- Rat
- Host
- Goat
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- 4-1BB/TNFRSF9/CD137 in Rat Splenocytes. 4-1BB/TNFRSF9/CD137 was detected in immersion fixed rat splenocytes treated with calcium ionomycin and PMA using Goat Anti-Rat 4-1BB/TNFRSF9/ CD137 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF9029) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of 4-1BB/TNFRSF9/CD137 in Rat Splenocytes by Flow Cytometry. Rat splenocytes either (A) resting or (B) activated with with 50 ng/mL PMA and 200 ng/mL Calcium Ionomycin overnight were stained with Goat Anti-Rat 4-1BB/TNFRSF9/CD137 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF9029) followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107) and APC-conjugated Anti-Rat CD3. Quadrant markers were set based on control antibody staining (Catalog # AB-108-C). View our protocol for Staining Membrane-associated Proteins.