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- Western blot [2]
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- Product number
- AF772 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Goat Polyclonal EMMPRIN/CD147 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects mouse EMMPRIN/CD147 in direct ELISAs and Western blots. In Western blots, approximately 5% cross-reactivity with recombinant human EMMPRIN is observed.
- Reactivity
- Mouse
- Host
- Goat
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references Basigin/CD147 promotes renal fibrosis after unilateral ureteral obstruction.
The E-selectin ligand basigin/CD147 is responsible for neutrophil recruitment in renal ischemia/reperfusion.
Basigin-mediated gene expression changes in mouse uterine stromal cells during implantation.
Evaluation of adenovirus-delivered human CD59 as a potential therapy for AMD in a model of human membrane attack complex formation on murine RPE.
Kato N, Kosugi T, Sato W, Ishimoto T, Kojima H, Sato Y, Sakamoto K, Maruyama S, Yuzawa Y, Matsuo S, Kadomatsu K
The American journal of pathology 2011 Feb;178(2):572-9
The American journal of pathology 2011 Feb;178(2):572-9
The E-selectin ligand basigin/CD147 is responsible for neutrophil recruitment in renal ischemia/reperfusion.
Kato N, Yuzawa Y, Kosugi T, Hobo A, Sato W, Miwa Y, Sakamoto K, Matsuo S, Kadomatsu K
Journal of the American Society of Nephrology : JASN 2009 Jul;20(7):1565-76
Journal of the American Society of Nephrology : JASN 2009 Jul;20(7):1565-76
Basigin-mediated gene expression changes in mouse uterine stromal cells during implantation.
Chen L, Belton RJ Jr, Nowak RA
Endocrinology 2009 Feb;150(2):966-76
Endocrinology 2009 Feb;150(2):966-76
Evaluation of adenovirus-delivered human CD59 as a potential therapy for AMD in a model of human membrane attack complex formation on murine RPE.
Ramo K, Cashman SM, Kumar-Singh R
Investigative ophthalmology & visual science 2008 Sep;49(9):4126-36
Investigative ophthalmology & visual science 2008 Sep;49(9):4126-36
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Mouse EMMPRIN/CD147 by Western Blot. Western blot shows lysates of mouse brain tissue, mouse heart tissue, and mouse small intestine tissue. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Mouse EMMPRIN/CD147 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF772) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for EMMPRIN/CD147 at approximately 45-60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Mouse EMMPRIN/CD147 by Simple WesternTM. Simple Western lane view shows lysates of mouse brain (cortex) tissue and mouse heart tissue, loaded at 0.2 mg/mL. A specific band was detected for EMMPRIN/CD147 at approximately 62 kDa (as indicated) using 2 µg/mL of Goat Anti-Mouse EMMPRIN/CD147 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF772) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
