Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Flow cytometry [1]
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- Product number
- 12-1472-42 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD147 Monoclonal Antibody (8D12), PE, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The monoclonal antibody 8D12 recognizes CD147 which is a member of the immunoglobulin superfamily. It is also called EMMPRIN and basigin. CD147 has been characterized as a tumor cell collagenase stimulating molecule and has also been shown to have a role in cell adhesion. CD147 can be found in association with integrins alpha3 beta1 and alpha6 beta1 by co-immpunopreciptations. Additionally it is thought to play a role in T cell activation and thymocyte cycling. CD147 is expressed broadly on hematopoietic and non-hematopoietic cells, especially in tumor cells. Applications Reported: This 8D12 antibody has been reported for use in flow cytometric analysis. Applications Tested: This 8D12 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Yellow dye
- Isotype
- IgG
- Antibody clone number
- 8D12
- Vial size
- 100 Tests
- Concentration
- 5 µL/Test
- Storage
- 4° C, store in dark, DO NOT FREEZE!
Submitted references Apheresis causes complement deposition on red blood cells (RBCs) and RBC antigen alterations, possibly inducing enhanced clearance.
Genetically engineered red cells expressing single domain camelid antibodies confer long-term protection against botulinum neurotoxin.
de Back DZ, Nezjad SG, Beuger BM, Veldhuis M, Clifford E, Ait Ichou F, Berghuis J, Go M, Gouwerok E, Meinderts S, Vrielink H, de Kort W, de Korte D, van Kraaij M, van Bruggen R
Transfusion 2018 Nov;58(11):2627-2634
Transfusion 2018 Nov;58(11):2627-2634
Genetically engineered red cells expressing single domain camelid antibodies confer long-term protection against botulinum neurotoxin.
Huang NJ, Pishesha N, Mukherjee J, Zhang S, Deshycka R, Sudaryo V, Dong M, Shoemaker CB, Lodish HF
Nature communications 2017 Sep 4;8(1):423
Nature communications 2017 Sep 4;8(1):423
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Staining of normal human peripheral blood cells with Mouse IgG1 kappa Isotype Control PE (Product # 12-4714-81) (open histogram) or Anti-Human CD147 PE (filled histogram). Cells in the lymphocyte gate (left) or monocyte gate (right) were used for analysis.
- Conjugate
- Yellow dye