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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Flow cytometry [2]
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- Product number
- TA501189 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA501189, RRID:AB_11141156
- Product name
- BSG (CD147) mouse monoclonal antibody, clone OTI10E10 (formerly 10E10)
- Antibody type
- Monoclonal
- Description
- BSG (CD147) mouse monoclonal antibody, clone OTI10E10 (formerly 10E10)
- Reactivity
- Canine
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- BSG
- Isotype
- IgG
- Antibody clone number
- OTI10E10
- Vial size
- 100 µl
- Concentration
- 1.00mg/ml
Submitted references Comparative proteomics of a model MCF10A-KRasG12V cell line reveals a distinct molecular signature of the KRasG12V cell surface.
Ye X, Chan KC, Waters AM, Bess M, Harned A, Wei BR, Loncarek J, Luke BT, Orsburn BC, Hollinger BD, Stephens RM, Bagni R, Martinko A, Wells JA, Nissley DV, McCormick F, Whiteley G, Blonder J
Oncotarget 2016 Dec 27;7(52):86948-86971
Oncotarget 2016 Dec 27;7(52):86948-86971
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-BSG monoclonal antibody.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BSG (RC219464, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BSG.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunofluorescent staining of HeLa cells using anti-BSG mouse monoclonal antibody (TA501189).
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Flow cytometric Analysis of Hela cells, using anti-BSG antibody(TA501189),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
- Validation comment
- FC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Flow cytometric Analysis of Jurkat cells, using anti-BSG antibody(TA501189),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
- Validation comment
- FC
