MA5-29060

antibody from Invitrogen Antibodies

Targeting: BSG CD147, EMMPRIN, EMPRIN, OK

Western blot

ELISA

Antibody data

Submit

Validation data

Product number: MA5-29060 - Provider product page
Provider: Invitrogen Antibodies
Product name: CD147 Recombinant Rabbit Monoclonal Antibody (125)
Antibody type: Monoclonal
Antigen: Recombinant full-length protein
Description: This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%). Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire. This antibody has specificity for Human CD147/Basigin/BSG.
Reactivity: Human
Host: Rabbit
Isotype: IgG
Antibody clone number: 125
Vial size: 100 µL
Concentration: 1 mg/mL
Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

BSG protein structure - MA5-29060 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry (Frozen) staining of CD147 in monkey stomach with CD147 Recombinant Rabbit Monoclonal Antibody (125) (Product # MA5-29060) (1:1,000, frozen section). The image showing membrane staining of gastric gland cell. Right panel: merge with DAPI.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemical staining of human CD147 in human esophagus with CD147 Recombinant Rabbit Monoclonal Antibody (125) (Product # MA5-29060, 1:5,000, formalin-fixed paraffin embedded sections). The image showing membrane staining of squamous epithelium cell. Left panel: tissue incubated with primary antibody. Right panel: tissue incubated with mixture of primary antibody and antigen (recombinant protein).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemical staining of human CD147 in human gastric cancer with CD147 Recombinant Rabbit Monoclonal Antibody (125) (Product # MA5-29060, 1:5,000, formalin-fixed paraffin embedded sections). The image showing membrane staining of epithelium cell.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemical staining of human CD147 in human liver with CD147 Recombinant Rabbit Monoclonal Antibody (125) (Product # MA5-29060, 1:5,000, formalin-fixed paraffin embedded sections). The image showing membrane staining of hepatocyte.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemical staining of human CD147 in human placenta with CD147 Recombinant Rabbit Monoclonal Antibody (125) (Product # MA5-29060, 1:5,000, formalin-fixed paraffin embedded sections). The image showing Positive staining trophoblast.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemical staining of human CD147 in human stomach with CD147 Recombinant Rabbit Monoclonal Antibody (125) (Product # MA5-29060, 1:5,000, formalin-fixed paraffin embedded sections). The image showing membrane staining of epithelium cell.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemical staining of human CD147 in human rectal cancer with CD147 Recombinant Rabbit Monoclonal Antibody (125) (Product # MA5-29060, 1:5,000, formalin-fixed paraffin embedded sections). The image showing membrane staining of epithelium cell in intestinal gland.