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LearnMA5-29060
antibody from Invitrogen Antibodies
Targeting: BSG
CD147, EMMPRIN, EMPRIN, OK
Western blot
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Immunocytochemistry Immunoprecipitation Immunohistochemistry Flow cytometry Other assayAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Immunocytochemistry [1]
- Flow cytometry [1]
- Other assay [1]
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- Product number
- MA5-29060 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD147 Recombinant Rabbit Monoclonal Antibody (125)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%). Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire. This antibody has specificity for Human CD147/Basigin/BSG.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- 125
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence staining of human CD147 in HeLa cells with rabbit monoclonal antibody (1:200). The image showing membrane staining of HeLa cells. CD147 Recombinant Rabbit Monoclonal Antibody (125) (Product # MA5-29060).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry analysis of anti-CD147 reactivity on HeLa cells using CD147 monoclonal antibody (Product # MA5-29060).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- CD147 Immunoprecipitation using: Lane A: 0.5 mg K562 Whole Cell Lysate 2 µL with CD147 Recombinant Rabbit Monoclonal Antibody (125) (Product # MA5-29060) and 15 µL of 50 % Protein G agarose. Primary antibody: CD147 Recombinant Rabbit Monoclonal Antibody (125), at 1:100 dilution. Secondary antibody: Clean-Bloto IP Detection Reagent (HRP) at 1:1,000 dilution. Developed using the DAB staining technique. Performed under reducing conditions. Predicted band size: 42 kDa. Observed band size: 50 kDa.
