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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- PA5-27296 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD147 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: K562 membrane extract.
- Concentration
- 1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot of CD147/BSG in non-transfected lysate 293T lysate (A) and transfected lysate (B). Samples were resolved on 12% SDS PAGE gel and probed using a CD147/BSG polyclonal antibody (Product # PA5-27296) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of CD147 was performed by separating 30 µg of K562 whole cell and membrane extracts by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a CD147 Polyclonal Antibody (Product # PA5-27296) at a dilution of 1:500.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded HSC3 xenograft, using CD147 (Product # PA5-27296) antibody at 1:100 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
