AP32095PU-S

antibody from Acris Antibodies GmbH

Targeting: GATA1 ERYF1, GATA-1, GF1, NF-E1, NFE1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

AP32095PU-S

Provider

Acris Antibodies GmbH

Product name

anti GATA1

Antibody type

Polyclonal

Antigen

N-terminal truncated Human recombinant protein.

Reactivity

Human, Mouse

Host

Rabbit

Vial size

20 µl

Concentration

1.0 mg/ml

Western blot

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Western blot analysis of GATA1 was performed by loading 25ug of various whole cell lysate onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% BSA/TBST for at least 1 hour. Membranes were then probed with a Rabbit polyclonal antibody recognizing GATA1 (Cat.-No AP32095PU-S) at a dilution of 1/500 overnight at 4°C on a rocking platform. Membranes were then washed in TBS 0.1% Tween-20 and probed with a Goat anti-Rabbit HRP secondary antibody.

Immunocytochemistry

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Immunofluorescent analysis of GATA1 (green) in U2OS cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature. Cells were then blocked with 5% normal goat serum for 15 minutes at room temperature. Cells were then probed with a Rabbit polyclonal antibody recognizing GATA1 (Cat.-No AP32095PU-S) at a dilution of 1/200 for at least hour at 37°C. Cells were then washed with PBS and incubated withb DyLight-488 Goat anti Rabbit secondary antibody at a dilution of 1/200 for 30 minutes at RT.