AF1779

antibody from R&D Systems

Targeting: GATA1 ERYF1, GATA-1, GF1, NF-E1, NFE1

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

AF1779

Provider

R&D Systems

Product name

Human GATA-1 Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. Detects human GATA-1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, this antibody shows less than 1% cross-reactivity with rhGATA-2, rhGATA-5 and rhGATA-6.

Reactivity

Human

Host

Goat

Conjugate

Unconjugated

Antigen sequence

P15976


Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Western blot

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of Human GATA-1 by Western Blot. Western blot shows lysates of K562 human chronic myelogenous leukemia cell line and TS1 mouse helper T cell line. PVDF membrane was probed with 0.5 µg/mL of Human GATA-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1779) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for GATA-1 at approximately 50 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1.