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Flow cytometryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Flow cytometry [1]
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- Product number
- IC1779P - Provider product page
- Provider
- R&D Systems
- Product name
- Human GATA-1 PE-conjugated Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human GATA-1 in direct ELISAs. In Western blots, this antibody does not cross-react with recombinant human (rh) GATA-2 or rhGATA-6.
- Reactivity
- Human
- Host
- Rat
- Antigen sequence
P15976- Isotype
- IgG
- Antibody clone number
- 234732
- Vial size
- 100 Tests
- Storage
- Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied.
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Detection of GATA-1 in MG-63 Human Cell Line by Flow Cytometry. MG-63 human osteosarcoma cell line was stained with Rat Anti-Human GATA-1 PE-conjugated Monoclonal Antibody (Catalog # IC1779P, filled histogram) or isotype control antibody (Catalog # IC013P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
