AF1607

antibody from Novus Biologicals

Targeting: CD163 M130, MM130, SCARI1

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

AF1607

Provider

Novus Biologicals

Product name

Goat Polyclonal CD163 Antibody

Antibody type

Polyclonal

References

Submitted references

Preferential use of Siglec-1 or Siglec-10 by type 1 and type 2 PRRSV strains to infect PK15^S1-CD163 and PK15^S10-CD163 cells.

Xie J, Christiaens I, Yang B, Trus I, Devriendt B, Cui T, Wei R, Nauwynck HJ
Veterinary research 2018 Jul 18;49(1):67

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Stabilin-1 is expressed in human breast cancer and supports tumor growth in mammary adenocarcinoma mouse model.

Riabov V, Yin S, Song B, Avdic A, Schledzewski K, Ovsiy I, Gratchev A, Llopis Verdiell M, Sticht C, Schmuttermaier C, Schönhaber H, Weiss C, Fields AP, Simon-Keller K, Pfister F, Berlit S, Marx A, Arnold B, Goerdt S, Kzhyshkowska J
Oncotarget 2016 May 24;7(21):31097-110

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Simian hemorrhagic fever virus cell entry is dependent on CD163 and uses a clathrin-mediated endocytosis-like pathway.

Caì Y, Postnikova EN, Bernbaum JG, Yú SQ, Mazur S, Deiuliis NM, Radoshitzky SR, Lackemeyer MG, McCluskey A, Robinson PJ, Haucke V, Wahl-Jensen V, Bailey AL, Lauck M, Friedrich TC, O'Connor DH, Goldberg TL, Jahrling PB, Kuhn JH
Journal of virology 2015 Jan;89(1):844-56

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CD163 and IgG codefend against cytotoxic hemoglobin via autocrine and paracrine mechanisms.

Subramanian K, Du R, Tan NS, Ho B, Ding JL
Journal of immunology (Baltimore, Md. : 1950) 2013 May 15;190(10):5267-78

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Efficient clearance of early apoptotic cells by human macrophages requires M2c polarization and MerTK induction.

Zizzo G, Hilliard BA, Monestier M, Cohen PL
Journal of immunology (Baltimore, Md. : 1950) 2012 Oct 1;189(7):3508-20

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Interaction of the European genotype porcine reproductive and respiratory syndrome virus (PRRSV) with sialoadhesin (CD169/Siglec-1) inhibits alveolar macrophage phagocytosis.

De Baere MI, Van Gorp H, Delputte PL, Nauwynck HJ
Veterinary research 2012 May 25;43:47

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Identification of the CD163 protein domains involved in infection of the porcine reproductive and respiratory syndrome virus.

Van Gorp H, Van Breedam W, Van Doorsselaere J, Delputte PL, Nauwynck HJ
Journal of virology 2010 Mar;84(6):3101-5

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Availability of activated CD4+ T cells dictates the level of viremia in naturally SIV-infected sooty mangabeys.

Klatt NR, Villinger F, Bostik P, Gordon SN, Pereira L, Engram JC, Mayne A, Dunham RM, Lawson B, Ratcliffe SJ, Sodora DL, Else J, Reimann K, Staprans SI, Haase AT, Estes JD, Silvestri G, Ansari AA
The Journal of clinical investigation 2008 Jun;118(6):2039-49

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Sialoadhesin and CD163 join forces during entry of the porcine reproductive and respiratory syndrome virus.

Van Gorp H, Van Breedam W, Delputte PL, Nauwynck HJ
The Journal of general virology 2008 Dec;89(Pt 12):2943-2953

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CD163 expression confers susceptibility to porcine reproductive and respiratory syndrome viruses.

Calvert JG, Slade DE, Shields SL, Jolie R, Mannan RM, Ankenbauer RG, Welch SK
Journal of virology 2007 Jul;81(14):7371-9

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Detection of Human CD163 by Western Blot. Western blot shows lysates of human thymus tissue, human liver tissue, and human placenta tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human CD163 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1607) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for CD163 at approximately 150 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Detection of Human CD163 by Simple Western^TM. Simple Western lane view shows lysates of human placenta tissue, loaded at 0.2 mg/mL. A specific band was detected for CD163 at approximately 179 kDa (as indicated) using 10 µg/mL of Goat Anti-Human CD163 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1607) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.