TA506390

antibody from OriGene

Targeting: CD163 M130, MM130, SCARI1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

TA506390

Provider

OriGene

Proper citation

OriGene Cat#TA506390, RRID:AB_2623749

Product name

CD163 mouse monoclonal antibody, clone OTI2C1 (formerly 2C1)

Antibody type

Monoclonal

Description

CD163 mouse monoclonal antibody, clone OTI2C1 (formerly 2C1)

Host

Mouse

Conjugate

Unconjugated

Epitope

CD163

Isotype

IgG

Antibody clone number

OTI2C1

Vial size

100 µl

Concentration

1.00mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-CD163 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

Validation comment

WB

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD163 (RC208557, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD163.

Validation comment

WB

Immunocytochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Anti-CD163 mouse monoclonal antibody (TA506390) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CD163(RC208557).

Validation comment

IF

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunofluorescent staining of HeLa cells using anti-CD163 mouse monoclonal antibody (TA506390).

Validation comment

IF