Antibody data
- Antibody Data
- Antigen structure
- References [14]
- Comments [0]
- Validations
- Flow cytometry [1]
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- Product number
- 14-1639-80 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-CD163 Monoclonal Antibody (eBioGHI/61 (GHI/61)), eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The eBioGHI/61 monoclonal antibody reacts with human CD163 (Haemoglobin Scavenger Receptor, M130), a member of the macrophage scavenger receptor family. CD163 is expressed by tissue macrophages, circulating blood monocytes, and some reports suggest that it may also be expressed by haematopoietic progenitor cells. CD163 functions as a high affinity scavenger receptor for the complex of haemoglobin and haptoglobin which forms to clear haemoglobin from the blood. The high affinity for CD163 is specific for the haemoglobin-haptoglobin complex, whereas haemoglobin or haptoglobin display no to low affinity toward CD163. Upon ligand binding, CD163 initiates signal transduction which leads to the production of IL-6 and IL-10. Furthermore, it has been demonstrated that cytokines such as IL-6 and IL-10 can induce expression of CD163, whereas proinflammatory stimuli, such as LPS or TNF alpha, can cause shedding of a soluble form of CD163, whose function is unclear. Applications Reported: This eBioGHI/61 (GHI/61) antibody has been reported for use in flow cytometric analysis, and immunoblotting (WB). Applications Tested: This eBioGHI/61 (GHI/61) antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- eBioGHI/61 (GHI/61)
- Vial size
- 25 µg
- Concentration
- 0.5 mg/mL
- Storage
- 4° C
Submitted references Recruitment of monocytes and epigenetic silencing of intratumoral CYP7B1 primarily contribute to the accumulation of 27-hydroxycholesterol in breast cancer.
TIM‑4 blockade of KCs combined with exogenous TGF‑β injection helps to reverse acute rejection and prolong the survival rate of mice receiving liver allografts.
Alternatively activated macrophages derived from THP-1 cells promote the fibrogenic activities of human dermal fibroblasts.
Immortalization of primary microglia: a new platform to study HIV regulation in the central nervous system.
Widespread Virus Replication in Alveoli Drives Acute Respiratory Distress Syndrome in Aerosolized H5N1 Influenza Infection of Macaques.
MicroRNA-720 suppresses M2 macrophage polarization by targeting GATA3.
The HIV-1 envelope protein gp120 is captured and displayed for B cell recognition by SIGN-R1(+) lymph node macrophages.
Microenvironments in tuberculous granulomas are delineated by distinct populations of macrophage subsets and expression of nitric oxide synthase and arginase isoforms.
Interleukin-25 fails to activate STAT6 and induce alternatively activated macrophages.
Heat shock protein 27 differentiates tolerogenic macrophages that may support human breast cancer progression.
Expression of the hemoglobin-haptoglobin receptor CD163 on hematopoietic progenitors.
Identification of the integral membrane protein RM3/1 on human monocytes as a glucocorticoid-inducible member of the scavenger receptor cysteine-rich family (CD163).
A new macrophage differentiation antigen which is a member of the scavenger receptor superfamily.
A monocyte/macrophage antigen recognized by the four antibodies GHI/61, Ber-MAC3, Ki-M8 and SM4.
Shi SZ, Lee EJ, Lin YJ, Chen L, Zheng HY, He XQ, Peng JY, Noonepalle SK, Shull AY, Pei FC, Deng LB, Tian XL, Deng KY, Shi H, Xin HB
American journal of cancer research 2019;9(10):2194-2208
American journal of cancer research 2019;9(10):2194-2208
TIM‑4 blockade of KCs combined with exogenous TGF‑β injection helps to reverse acute rejection and prolong the survival rate of mice receiving liver allografts.
Wu H, Xu X, Li J, Gong J, Li M
International journal of molecular medicine 2018 Jul;42(1):346-358
International journal of molecular medicine 2018 Jul;42(1):346-358
Alternatively activated macrophages derived from THP-1 cells promote the fibrogenic activities of human dermal fibroblasts.
Zhu Z, Ding J, Ma Z, Iwashina T, Tredget EE
Wound repair and regeneration : official publication of the Wound Healing Society [and] the European Tissue Repair Society 2017 May;25(3):377-388
Wound repair and regeneration : official publication of the Wound Healing Society [and] the European Tissue Repair Society 2017 May;25(3):377-388
Immortalization of primary microglia: a new platform to study HIV regulation in the central nervous system.
Garcia-Mesa Y, Jay TR, Checkley MA, Luttge B, Dobrowolski C, Valadkhan S, Landreth GE, Karn J, Alvarez-Carbonell D
Journal of neurovirology 2017 Feb;23(1):47-66
Journal of neurovirology 2017 Feb;23(1):47-66
Widespread Virus Replication in Alveoli Drives Acute Respiratory Distress Syndrome in Aerosolized H5N1 Influenza Infection of Macaques.
Wonderlich ER, Swan ZD, Bissel SJ, Hartman AL, Carney JP, O'Malley KJ, Obadan AO, Santos J, Walker R, Sturgeon TJ, Frye LJ Jr, Maiello P, Scanga CA, Bowling JD, Bouwer AL, Duangkhae PA, Wiley CA, Flynn JL, Wang J, Cole KS, Perez DR, Reed DS, Barratt-Boyes SM
Journal of immunology (Baltimore, Md. : 1950) 2017 Feb 15;198(4):1616-1626
Journal of immunology (Baltimore, Md. : 1950) 2017 Feb 15;198(4):1616-1626
MicroRNA-720 suppresses M2 macrophage polarization by targeting GATA3.
Zhong Y, Yi C
Bioscience reports 2016 Aug;36(4)
Bioscience reports 2016 Aug;36(4)
The HIV-1 envelope protein gp120 is captured and displayed for B cell recognition by SIGN-R1(+) lymph node macrophages.
Park C, Arthos J, Cicala C, Kehrl JH
eLife 2015 Aug 10;4
eLife 2015 Aug 10;4
Microenvironments in tuberculous granulomas are delineated by distinct populations of macrophage subsets and expression of nitric oxide synthase and arginase isoforms.
Mattila JT, Ojo OO, Kepka-Lenhart D, Marino S, Kim JH, Eum SY, Via LE, Barry CE 3rd, Klein E, Kirschner DE, Morris SM Jr, Lin PL, Flynn JL
Journal of immunology (Baltimore, Md. : 1950) 2013 Jul 15;191(2):773-84
Journal of immunology (Baltimore, Md. : 1950) 2013 Jul 15;191(2):773-84
Interleukin-25 fails to activate STAT6 and induce alternatively activated macrophages.
Stolfi C, Caruso R, Franzè E, Sarra M, De Nitto D, Rizzo A, Pallone F, Monteleone G
Immunology 2011 Jan;132(1):66-77
Immunology 2011 Jan;132(1):66-77
Heat shock protein 27 differentiates tolerogenic macrophages that may support human breast cancer progression.
Banerjee S, Lin CF, Skinner KA, Schiffhauer LM, Peacock J, Hicks DG, Redmond EM, Morrow D, Huston A, Shayne M, Langstein HN, Miller-Graziano CL, Strickland J, O'Donoghue L, De AK
Cancer research 2011 Jan 15;71(2):318-27
Cancer research 2011 Jan 15;71(2):318-27
Expression of the hemoglobin-haptoglobin receptor CD163 on hematopoietic progenitors.
Matthews KE, Mueller SG, Woods C, Bell DN
Stem cells and development 2006 Feb;15(1):40-8
Stem cells and development 2006 Feb;15(1):40-8
Identification of the integral membrane protein RM3/1 on human monocytes as a glucocorticoid-inducible member of the scavenger receptor cysteine-rich family (CD163).
Högger P, Dreier J, Droste A, Buck F, Sorg C
Journal of immunology (Baltimore, Md. : 1950) 1998 Aug 15;161(4):1883-90
Journal of immunology (Baltimore, Md. : 1950) 1998 Aug 15;161(4):1883-90
A new macrophage differentiation antigen which is a member of the scavenger receptor superfamily.
Law SK, Micklem KJ, Shaw JM, Zhang XP, Dong Y, Willis AC, Mason DY
European journal of immunology 1993 Sep;23(9):2320-5
European journal of immunology 1993 Sep;23(9):2320-5
A monocyte/macrophage antigen recognized by the four antibodies GHI/61, Ber-MAC3, Ki-M8 and SM4.
Pulford K, Micklem K, McCarthy S, Cordell J, Jones M, Mason DY
Immunology 1992 Apr;75(4):588-95
Immunology 1992 Apr;75(4):588-95
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Supportive validation
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- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Staining of normal human peripheral blood cells with 0.25 µg of Mouse IgG1 K Isotype Control Purified (Product # 14-4714-82) (open histogram) or 0.25 µg of Anti-Human CD163 Purified (filled histogram) followed by Anti-Mouse IgG FITC (Product # 11-4011-85). Cells in the monocyte gate were used for analysis.