14-1639-80

antibody from Invitrogen Antibodies
Targeting: CD163 M130, MM130, SCARI1
Provider product page for 14-1639-80
Western blot
Flow cytometry

Antibody data

Product number
14-1639-80 - Provider product page
Provider
Invitrogen Antibodies
Product name
Anti-CD163 Monoclonal Antibody (eBioGHI/61 (GHI/61)), eBioscience™
Antibody type
Monoclonal
Antigen
Other
Description
Description: The eBioGHI/61 monoclonal antibody reacts with human CD163 (Haemoglobin Scavenger Receptor, M130), a member of the macrophage scavenger receptor family. CD163 is expressed by tissue macrophages, circulating blood monocytes, and some reports suggest that it may also be expressed by haematopoietic progenitor cells. CD163 functions as a high affinity scavenger receptor for the complex of haemoglobin and haptoglobin which forms to clear haemoglobin from the blood. The high affinity for CD163 is specific for the haemoglobin-haptoglobin complex, whereas haemoglobin or haptoglobin display no to low affinity toward CD163. Upon ligand binding, CD163 initiates signal transduction which leads to the production of IL-6 and IL-10. Furthermore, it has been demonstrated that cytokines such as IL-6 and IL-10 can induce expression of CD163, whereas proinflammatory stimuli, such as LPS or TNF alpha, can cause shedding of a soluble form of CD163, whose function is unclear. Applications Reported: This eBioGHI/61 (GHI/61) antibody has been reported for use in flow cytometric analysis, and immunoblotting (WB). Applications Tested: This eBioGHI/61 (GHI/61) antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 µm post-manufacturing filtered.
Reactivity
Human
Host
Mouse
Isotype
IgG
Antibody clone number
eBioGHI/61 (GHI/61)
Vial size
25 µg
Concentration
0.5 mg/mL
Storage
4° C
CD163 protein structure - 14-1639-80 shown in red.