Antibody data
- Antibody Data
- Antigen structure
- References [8]
- Comments [0]
- Validations
- Flow cytometry [1]
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- Product number
- 46-1639-41 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-CD163 Monoclonal Antibody (eBioGHI/61 (GHI/61)), PerCP-eFluor 710, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The eBioGHI/61 monoclonal antibody reacts with human CD163 (Haemoglobin Scavenger Receptor, M130), a member of the macrophage scavenger receptor family. CD163 is expressed by tissue macrophages, circulating blood monocytes, and some reports suggest that it may also be expressed by haematopoietic progenitor cells. CD163 functions as a high affinity scavenger receptor for the complex of haemoglobin and haptoglobin which forms to clear haemoglobin from the blood. The high affinity for CD163 is specific for the haemoglobin-haptoglobin complex, whereas haemoglobin or haptoglobin display no to low affinity toward CD163. Upon ligand binding, CD163 initiates signal transduction which leads to the production of IL-6 and IL-10. Furthermore, it has been demonstrated that cytokines such as IL-6 and IL-10 can induce expression of CD163, whereas proinflammatory stimuli, such as LPS or TNF alpha, can cause shedding of a soluble form of CD163, whose function is unclear. Applications Reported: This eBioGHI/61 (GHI/61) antibody has been reported for use in flow cytometric analysis. Applications Tested: This eBioGHI/61 (GHI/61) antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.5 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5. We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative. Please make sure that your instrument is capable of detecting this fluorochrome. Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically. Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- eBioGHI/61 (GHI/61)
- Vial size
- 25 Tests
- Concentration
- 5 µL/Test
- Storage
- 4° C, store in dark, DO NOT FREEZE!
Submitted references Recruitment of monocytes and epigenetic silencing of intratumoral CYP7B1 primarily contribute to the accumulation of 27-hydroxycholesterol in breast cancer.
TIM‑4 blockade of KCs combined with exogenous TGF‑β injection helps to reverse acute rejection and prolong the survival rate of mice receiving liver allografts.
Alternatively activated macrophages derived from THP-1 cells promote the fibrogenic activities of human dermal fibroblasts.
Immortalization of primary microglia: a new platform to study HIV regulation in the central nervous system.
MicroRNA-720 suppresses M2 macrophage polarization by targeting GATA3.
Interleukin-25 fails to activate STAT6 and induce alternatively activated macrophages.
A new macrophage differentiation antigen which is a member of the scavenger receptor superfamily.
A monocyte/macrophage antigen recognized by the four antibodies GHI/61, Ber-MAC3, Ki-M8 and SM4.
Shi SZ, Lee EJ, Lin YJ, Chen L, Zheng HY, He XQ, Peng JY, Noonepalle SK, Shull AY, Pei FC, Deng LB, Tian XL, Deng KY, Shi H, Xin HB
American journal of cancer research 2019;9(10):2194-2208
American journal of cancer research 2019;9(10):2194-2208
TIM‑4 blockade of KCs combined with exogenous TGF‑β injection helps to reverse acute rejection and prolong the survival rate of mice receiving liver allografts.
Wu H, Xu X, Li J, Gong J, Li M
International journal of molecular medicine 2018 Jul;42(1):346-358
International journal of molecular medicine 2018 Jul;42(1):346-358
Alternatively activated macrophages derived from THP-1 cells promote the fibrogenic activities of human dermal fibroblasts.
Zhu Z, Ding J, Ma Z, Iwashina T, Tredget EE
Wound repair and regeneration : official publication of the Wound Healing Society [and] the European Tissue Repair Society 2017 May;25(3):377-388
Wound repair and regeneration : official publication of the Wound Healing Society [and] the European Tissue Repair Society 2017 May;25(3):377-388
Immortalization of primary microglia: a new platform to study HIV regulation in the central nervous system.
Garcia-Mesa Y, Jay TR, Checkley MA, Luttge B, Dobrowolski C, Valadkhan S, Landreth GE, Karn J, Alvarez-Carbonell D
Journal of neurovirology 2017 Feb;23(1):47-66
Journal of neurovirology 2017 Feb;23(1):47-66
MicroRNA-720 suppresses M2 macrophage polarization by targeting GATA3.
Zhong Y, Yi C
Bioscience reports 2016 Aug;36(4)
Bioscience reports 2016 Aug;36(4)
Interleukin-25 fails to activate STAT6 and induce alternatively activated macrophages.
Stolfi C, Caruso R, Franzè E, Sarra M, De Nitto D, Rizzo A, Pallone F, Monteleone G
Immunology 2011 Jan;132(1):66-77
Immunology 2011 Jan;132(1):66-77
A new macrophage differentiation antigen which is a member of the scavenger receptor superfamily.
Law SK, Micklem KJ, Shaw JM, Zhang XP, Dong Y, Willis AC, Mason DY
European journal of immunology 1993 Sep;23(9):2320-5
European journal of immunology 1993 Sep;23(9):2320-5
A monocyte/macrophage antigen recognized by the four antibodies GHI/61, Ber-MAC3, Ki-M8 and SM4.
Pulford K, Micklem K, McCarthy S, Cordell J, Jones M, Mason DY
Immunology 1992 Apr;75(4):588-95
Immunology 1992 Apr;75(4):588-95
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Staining of normal human peripheral blood cells with Mouse IgG1 K Isotype Control PerCP-eFluor® 710 (Product # 46-4714-82) (blue histogram) or Anti-Human CD163 PerCP-eFluor® 710 (purple histogram). Cells in the monocyte gate were used for analysis.