326502

antibody from BioLegend

Targeting: CD163 M130, MM130, SCARI1

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

326502

Provider

BioLegend

Proper citation

BioLegend Cat#326502, RRID:AB_893267

Product name

Purified anti-human CD163

Antibody type

Monoclonal

Antigen

human monocytes

Description

CD163 is a member of the group B scavenger receptor cysteine-rich superfamily, also known as GHI/61, M130, RM3/1, p155, Hemoglobin-Haptoglobin Complex Receptor, or macrophage-associated antigen. It is a 134 kD (non-reduced)/155 kD (reduced) glycoprotein primarily expressed on macrophages, Kuffer cells, monocytes, subset of dendritic cells, and a subset of hematopoietic stem/progenitor cells. CD163 binds to haptoglobin-hemoglobin complex and TWEAK, and plays a role in clearing hemoglobin and regulating cytokine production by macrophages. Membrane CD163 can be cleaved by metalloproteinases (MMP) resulting in soluble form. Elevated serum level of sCD163 has been implicated in many kinds of inflammation diseases.

Reactivity

Human

Host

Mouse

Conjugate

Unconjugated

Isotype

IgG

Vial size

100 µg

Concentration

0.5 mg/ml

Storage

The antibody solution should be stored undiluted at 4°C.

References

Submitted references

Basal-like Breast Cancer Cells Induce Phenotypic and Genomic Changes in Macrophages.

Stewart DA, Yang Y, Makowski L, Troester MA
Molecular cancer research : MCR 2012 Jun;10(6):727-38

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Folate receptor beta is expressed by tumor-associated macrophages and constitutes a marker for M2 anti-inflammatory/regulatory macrophages.

Puig-Kröger A, Sierra-Filardi E, Domínguez-Soto A, Samaniego R, Corcuera MT, Gómez-Aguado F, Ratnam M, Sánchez-Mateos P, Corbí AL
Cancer research 2009 Dec 15;69(24):9395-403

Flow cytometry

Supportive validation

Submitted by

BioLegend (provider)

Main image

Experimental details

GM-CSF-stimulated (day-3) human monocytes stained with purified RM3/1 , followed by anti-mouse IgG FITC

Conjugate

Unconjugated