MA5-11458

antibody from Invitrogen Antibodies

Targeting: CD163 M130, MM130, SCARI1

Provider product page for MA5-11458

Western blot

Immunohistochemistry

Other assay

Antibody data

Antibody Data
Antigen structure
References [44]
Comments [0]
Validations
Western blot [1]
Immunohistochemistry [1]
Other assay [6]

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Product number: MA5-11458 - Provider product page
Provider: Invitrogen Antibodies
Product name: CD163 Monoclonal Antibody (10D6)
Antibody type: Monoclonal
Antigen: Recombinant full-length protein
Description: MA5-11458 targets CD163 in WB and IHC (P) applications and shows reactivity with Human samples. The MA5-11458 immunogen is recombinant protein encoding of domains 1-4 of the N-terminal region of human CD163.
Reactivity: Human
Host: Mouse
Isotype: IgG
Antibody clone number: 10D6
Vial size: 500 µL
Concentration: Conc. Not Determined
Storage: 4° C

CD163 protein structure - MA5-11458 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 1 Human monocytes express FFAR2 and FFAR3 which are downregulated following differentiation into macrophages. Human tissue sections were probed via immunohistochemistry. The merged color (blue), is the result of the colocalization of antibody staining for FFAR2 or FFAR3 (both stained green) with the CD163 monocytes/macrophage marker (stained red). ( a ) Colon epithelial cells, liver hepatocytes, and the CD163+ spleen monocytes/macrophages, stained positive for FFAR2 and FFA3. Only weak FFAR2 and FFAR3 staining was observed for the CD163+ monocytes/macrophages of the colon, liver, and lung. ( b ) CD163+ peripheral blood monocytes stained positive for FFAR2 and FFAR3, and this staining was reduced when a portion of the monocyte sample was differentiated in vitro into macrophages. The data shown are representative of three independent experiments.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 5 Multiplexed CyCIF Immunofluorescence Images Showing Macrophages in the Lungs of SARS-CoV-2-Challenged Macaques as well as Macrophages Transcriptional Signatures in BAL, Peripheral Blood, and Serum of Infected Macaques Multiplexed CyCIF images showing macrophage populations in large airways (A, D, and G) and in bronchioles and alveoli (B, E, and H) in regions of lung consolidation as compared to uninvolved lung on day 2 following SARS CoV-2 infection. Tissue architecture is defined by E-Cadherin (grayscale), smooth muscle actin (aSMA, magenta), and DNA (blue). (A-C) CD68 (green) and CD163 (red) are used to stain macrophages in the context of the tissue markers smooth muscle actin (alphaSMA, magenta). (D-F) Alveolar (CD206, red) and reactive (CD16, green and CD68, magenta) macrophage markers. (G-I) Staining for activated macrophages (IBA1, green). Scale bars, 200 muM. (J) Heatmaps presenting the sample-level enrichment analysis (SLEA) Z score of macrophages' signatures for each individual animal at baseline and on days 1, 2, 4, 7, 10, and 14 following SARS-CoV-2 infection in BAL (top panel) and in peripheral blood (lower panel). Columns are grouped by time point, where each column corresponds to an individual animal. Each row represents a signature of macrophages that is increased by SARS-CoV-2. An SLEA Z score greater than 0 corresponds to a pathway for which member genes are upregulated, whereas an SLEA Z score less than 0 corresponds to a pathway for which genes are

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Fig. 2 DNA methylation of the metabolism genes in macrophages is induced by direct interaction with PDA cells through GARP/TGF-betaRII-integrin alphaV/beta8. a , b Nqo-1 and Aldh1a3 methylation in mouse BMDMs in a transwell system separated from KPC cells by an 8-mum or 1-mum pore membrane that, respectively, allows or not allows tumor cells to migrate through and direct contract with macrophages and in BMDMs cultured with TCM. Nqo-1 and Aldh1a3 methylation quantified as described in Supplementary Methods. * P < 0.05 (Mann-Whitney U test). c Lucifer Yellow labeled-KPC cells were co-cultured with unlabeled BMDMs. Thick arrows indicate macrophages that contain Lucifer Yellow spread from KPC cells (thin arrow) around them. Scale bar: 20 mum. d GARP expression on M0, M1-like, and M2-like macrophages measured by immunofluorescent staining with FITC-conjugated anti-GARP antibody. Arrow indicates macrophages that have the highest fluorescence within each image. Scale bar: 20 mum. * P < 0.05 (ANOVA). Histogram (right panel) shows quantification of fluorescence intensity. e Multiplex immunohistochemistry (IHC) was performed on a single slide of human PDA tissues for GARP (in green), CD68 (in red) and CD163 (in purple). A representative among 20 human PDAs tested is shown. Arrows (both panels) indicate GARP-expressing CD68 + CD163 + (M2-like) macrophages ; and arrowheads (left panel) indicate GARP-expressing CD68 + CD163 - (M1-like) macrophages . Notched arrowheads (right panel) indica

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Negative correlation between ecto-enzymes and CCND1 expression in CRC. ( A ) IHC staining of human normal colon (n = 40) and colon adenocarcinoma (n = 64) specimens in a tissue microarray for CCND1 and CD163 (a macrophage marker). ( B ) Relative intensities of CCND1 staining in the lamina propria of a normal and cancerous colon. ** P < .01 (unpaired t test). ( C ) Correlation analysis of NT5E and CCND1 expression in the TCGA colorectal adenocarcinoma dataset. *** P < .001 (Student t test).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Fig. 6. Elevated IL10 expression and STAT3 activation in ADPKD kidney macrophages. (A) Elevated IL10 expression in human ADPKD kidneys. RNA was isolated from NHKs and non-cystic regions of ADPKD kidneys (three of each). IL10 expression was analyzed by performing qRT-PCR. The line indicates the mean relative value. (B) IL-10 is present in ADPKD cyst fluid. IL-10 in pooled cyst fluid collected from ADPKD kidneys from 12 different individuals was measured by using ELISA. Data are mean+-s.e.m. (C-G) Phospho-STAT3 was present in macrophages of cystic ADPKD kidneys. Formalin-fixed, paraffin-embedded tissues from ADPKD kidneys were serially sectioned (C-E), and consecutive sections were stained by using immunohistochemistry with antibodies against the M2 macrophage marker CD163 (C), phospho-STAT3 (D) or secondary antibody only (E, No Ab) as a control. Arrows indicate phospho-STAT3-positive cells in macrophage-rich regions of interstitium. (F,G) Sections of ADPKD kidneys that had been fixed and prepared in a similar manner were co-stained with anti-CD163 (green) and anti-phospho-STAT3 (red) antibodies, followed by incubation with two distinct fluorescence-labeled secondary antibodies, each with the appropriate antibody specificity (F), and with DAPI (G). Arrowheads indicate co-staining cells. n =3 biological replicates. hIL-10, human IL-10.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 6 Accumulation of functional monocytes in proximity to follicular areas during SIV infection. ( A ) Representative flow cytometric plots showing the gating scheme for identification of monocyte subsets and pooled data showing the relative frequency of CD14 hi CD16 hi monocytes in LNs from noninfected RMs ( n = 11), chronically infected RMs ( n = 11), and chronically infected AGMs ( n = 5). * P < 0.05, by Mann-Whitney U test. ( B ) Linear regression analysis showing the association between the frequency of LN CD14 hi CD16 hi monocytes and LN total CD8 + T cells. ( C ) Representative confocal images showing the distribution of monocytes (CD163 hi , in red) and granulocytes (MPO hi , in green) in LN tissues from noninfected and acutely and chronically SIV-infected RMs. Two zoomed areas close to the B cell follicle (defined by CD20 and Ki67 expression) from each animal are also shown. Scale bars: 400mum (top two), 200 mum (third row), and 300 mum (lower); enlarged 50 mum; 40 mum (second row, right). Original magnification, x20. ( D ) Pooled data showing CXCL10 production by CD14 hi CD16 hi and CD14 hi CD16 lo monocytes (flow cytometric intracellular staining analysis) after short in vitro stimulation with either IFN-alpha or IFN-gamma. Cells from noninfected ( n = 8) and chronically SIV-infected ( n = 8) RMs were analyzed. * P < 0.05, by Mann-Whitney U test. ( E ) Relative frequency of LN CD14 hi CD16 hi monocytes before and after cART from RMs tr