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ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [7]
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- Validations
- Immunohistochemistry [7]
- Flow cytometry [3]
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- Product number
- NB110-40686 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB110-40686, RRID:AB_714951
- Product name
- Mouse Monoclonal CD163 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A purified. NB110-40686 recognizes the human CD163 cell surface antigen, a 130-140kD glycoprotein expressed by tissue macrophages. CD163 is not expressed by resting peripheral blood leucocytes, but expression may be induced on monocytes by culture in dexamethasone.
- Reactivity
- Human, Bovine, Guinea Pig, Porcine, Sheep, Simian
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Impact of uterine macrophage phenotype on placental retention in dairy cows.
Antibody Cross-Linking of CD14 Activates MerTK and Promotes Human Macrophage Clearance of Apoptotic Neutrophils: the Dual Role of CD14 at the Crossroads Between M1 and M2c Polarization.
Unwavering Pathobiology of Volumetric Muscle Loss Injury.
Highly efficient maternal-fetal Zika virus transmission in pregnant rhesus macaques.
Bifidobacterium pseudocatenulatum CECT7765 induces an M2 anti-inflammatory transition in macrophages from patients with cirrhosis.
Phenotypic changes in the brain of SIV-infected macaques exposed to methamphetamine parallel macrophage activation patterns induced by the common gamma-chain cytokine system.
Circulating levels of soluble MER in lupus reflect M2c activation of monocytes/macrophages, autoantibody specificities and disease activity.
Nelli RK, De Koster J, Roberts JN, de Souza J, Lock AL, Raphael W, Agnew D, Contreras GA
Theriogenology 2019 Mar 15;127:145-152
Theriogenology 2019 Mar 15;127:145-152
Antibody Cross-Linking of CD14 Activates MerTK and Promotes Human Macrophage Clearance of Apoptotic Neutrophils: the Dual Role of CD14 at the Crossroads Between M1 and M2c Polarization.
Zizzo G, Cohen PL
Inflammation 2018 Dec;41(6):2206-2221
Inflammation 2018 Dec;41(6):2206-2221
Unwavering Pathobiology of Volumetric Muscle Loss Injury.
Greising SM, Rivera JC, Goldman SM, Watts A, Aguilar CA, Corona BT
Scientific reports 2017 Oct 13;7(1):13179
Scientific reports 2017 Oct 13;7(1):13179
Highly efficient maternal-fetal Zika virus transmission in pregnant rhesus macaques.
Nguyen SM, Antony KM, Dudley DM, Kohn S, Simmons HA, Wolfe B, Salamat MS, Teixeira LBC, Wiepz GJ, Thoong TH, Aliota MT, Weiler AM, Barry GL, Weisgrau KL, Vosler LJ, Mohns MS, Breitbach ME, Stewart LM, Rasheed MN, Newman CM, Graham ME, Wieben OE, Turski PA, Johnson KM, Post J, Hayes JM, Schultz-Darken N, Schotzko ML, Eudailey JA, Permar SR, Rakasz EG, Mohr EL, Capuano S 3rd, Tarantal AF, Osorio JE, O'Connor SL, Friedrich TC, O'Connor DH, Golos TG
PLoS pathogens 2017 May;13(5):e1006378
PLoS pathogens 2017 May;13(5):e1006378
Bifidobacterium pseudocatenulatum CECT7765 induces an M2 anti-inflammatory transition in macrophages from patients with cirrhosis.
Moratalla A, Caparrós E, Juanola O, Portune K, Puig-Kröger A, Estrada-Capetillo L, Bellot P, Gómez-Hurtado I, Piñero P, Zapater P, González-Navajas JM, Such J, Sanz Y, Francés R
Journal of hepatology 2016 Jan;64(1):135-45
Journal of hepatology 2016 Jan;64(1):135-45
Phenotypic changes in the brain of SIV-infected macaques exposed to methamphetamine parallel macrophage activation patterns induced by the common gamma-chain cytokine system.
Bortell N, Morsey B, Basova L, Fox HS, Marcondes MC
Frontiers in microbiology 2015;6:900
Frontiers in microbiology 2015;6:900
Circulating levels of soluble MER in lupus reflect M2c activation of monocytes/macrophages, autoantibody specificities and disease activity.
Zizzo G, Guerrieri J, Dittman LM, Merrill JT, Cohen PL
Arthritis research & therapy 2013;15(6):R212
Arthritis research & therapy 2013;15(6):R212
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Frozen: CD163 Antibody (EDHu-1) [NB110-40686] - Staining of a human tonsil cryosection with Mouse anti Human CD163 antibody, clone EDHu-1.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Frozen: CD163 Antibody (EDHu-1) [NB110-40686] - Staining of a human tonsil cryosection with Mouse anti Human CD163 antibody, clone EDHu-1.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Frozen: CD163 Antibody (EDHu-1) [NB110-40686] - Staining of a human tonsil cryosection with Mouse anti Human CD163 antibody, clone EDHu-1.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Frozen: CD163 Antibody (EDHu-1) [NB110-40686] - Human tonsil cryosection with Mouse anti Human CD163 antibody, clone, red in A and Mouse anti Human CD21 antibody, green in B. C is the merged image with nuclei counterstained blue using DAPI.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: CD163 Antibody (EDHu-1) [NB110-40686] - FFPE human tonsil, stained with anti-CD163, pH 9 antigen retrieval. IHC-i Image submitted by a verified customer review.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Frozen: CD163 Antibody (EDHu-1) [NB110-40686] - Frozen human skin sections. CD163 (green) in control human skin. Sections were fixed with 4% PFA PBS, permeabilized wtih 0.5% Triton TBS Ca/Azide, and blocked with a 5% BSA 0.05% T20 TBS Ca/Azide solution. Antibody was diluted 1:100 and applied for 1 hr at 37C. Detection was performed with anti-host conjugated antibody, and sections were counterstained with DAPI. IHC-Fr image submitted by a verified customer review.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: CD163 Antibody (EDHu-1) [NB110-40686] - Non-repaired and repaired VML injured muscles present sustained putative macrophage infiltration. Representative histologic images labeled with CD163 (pan macrophage marker) and wheat germ agglutinin (WGA) and DAPI (nuclei). For each of the sham muscle sections individual and merged images are presented. Image collected and cropped by CiteAb from the following publication (http://www.nature.com/articles/s41598-017-13306-2), licensed under a CC-BY licence.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow (Cell Surface): CD163 Antibody (EDHu-1) [NB110-40686] - A surface stain was performed on human peripheral blood monocytes with CD163 (EDHu-1) antibody NB110-40686APC and a matched isotype control NBP2-27287APC. Cells were incubated in an antibody dilution of 1 ug/mL for 20 minutes at room temperature. A co-stain was performed with NB100-77758AF488.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: CD163 Antibody (EDHu-1) [NB110-40686] - A surface stain was performed on hPBMCs with CD163 (EDHu-1) antibody NB110-40686AF700 and a matched isotype control NBP2-27287AF700. Cells were incubated in an antibody dilution of 5 ug/mL for 20 minutes at room temperature. A co-stain was performed with NB100-77758AF488. Image using the Alexa Fluor 700 form of this antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow (Cell Surface): CD163 Antibody (EDHu-1) [NB110-40686] - A surface stain was performed on hPBMCs with CD163 (EDHu-1) antibody NB110-40686PE and a matched isotype control NBP2-27287PE. Cells were incubated in an antibody dilution of 2.5 ug/mL for 20 minutes at room temperature. A co-stain was performed with NB100-77758APC. Image using the PE form of this antibody.
