Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- PA5-14215 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD163 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µL
- Concentration
- 2 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references Iron-Overload triggers ADAM-17 mediated inflammation in Severe Alcoholic Hepatitis.
Maras JS, Das S, Sharma S, Sukriti S, Kumar J, Vyas AK, Kumar D, Bhat A, Yadav G, Choudhary MC, Sharma S, Kumar G, Bihari C, Trehanpati N, Maiwall R, Sarin SK
Scientific reports 2018 Jul 6;8(1):10264
Scientific reports 2018 Jul 6;8(1):10264
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis using a CD163 polyclonal antibody (Product # PA5-14215) in K562 cell lysates (35 µg per lane).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of formalin-fixed, paraffin-embedded human hepatocarcinoma tissue using a CD163 polyclonal antibody (Product # PA5-14215), followed by HRP-conjugated secondary antibody and DAB staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 2 Increased CD163 and ADAM17 expression is associated to iron-overload in SAH. ( A ) Workflow and summary of the experiments performed and number of samples used in target determination, and validation experiments including the In-vivo and In-vitro analysis. ( B ) Venn-diagram for unique and shared genes (p < 0.05, fold change >1.5) in the liver and PBMC transcriptome of Group A: SAH-IO.16 shared genes in the liver and PBMC were associated to cellular iron ion homeostasis, cytokine receptor binding and notch signaling. ( C ) Venn-diagram for unique and shared genes identified in the univariate and multivariate projection analysis for the liver and PBMC transcriptome of Group A: SAH-IO documenting CD163, ADAM17 and TMED7 significantly associated to systemic iron overload. ( D ) Immuno-histochemistry (IHC) for iron deposition (Perl's-staining) and expression of ADAM17, CD163, ADAM10 in Group A: SAH-IO (n = 5) vs. Group B: SAH-NIO (n = 5). [For all IHC analysis relative quantization of positively stained cells are expressed as mean number of positive cells/10 high power field (40x)]. ( E ) Perl's staining and expression of CD68, ADAM17, CD163 and ADAM10 in Group A: SAH-IO (n = 5) vs. AC (n = 5). ( F ) Perl's staining, and expression of ADAM17, CD163 and ADAM10 in Thalassemia (n = 3) patients compared to Group A and B.