PA1-10012

antibody from Invitrogen Antibodies

Targeting: PRPH NEF4, PRPH1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Recommended by provider)

Antibody Data

Product number

PA1-10012

Provider

Invitrogen Antibodies

Product name

Peripherin Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Purifed from natural sources

Description

Stable at 4°C for at least one year. Mix 1:1 with 100% glycerol and store at -20°C for longer term storage.

Reactivity

Human, Mouse, Rat, Bovine, Porcine

Host

Chicken/Avian

Isotype

IgY

Vial size

100 µL

Concentration

Conc. Not Determined

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. Glycerol (1:1) may be added for added stability.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of peripherin in cell and tissue lysates using a peripherin polyclonal antibody (Product # PA1-10012) at a dilution of 1:10,000 as seen in green. 1) protein standard (red), 2) rat spinal cord tissue, 3) mouse spinal cord tissue, 4) pig spinal cord tissue, 5) cow spinal cord; 6) SH-SY5Y, and 7) PC12 cells. The band at 57kDa corresponds to peripherin protein.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of Peripherin in mixed fibroblast and PC12 pheochromocytoma cell culture. Samples were stained using a Peripherin polyclonal antibody (Product # PA1-10012) at a dilution of 1:5,000 as seen in green, costained using a vimentin polyclonal antibody at a dilution 1:2,000 as seen in red, and with DAPI staining the nuclear DNA in blue. The Peripherin antibody reveals cytoplasmic filamentous staining in the small PC12 cells, while vimentin antibody stains intermediate filaments in the surrounding fibroblastic cells which do not express peripherin.