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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
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Validation data
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- Product number
- PA1-10012 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Peripherin Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Purifed from natural sources
- Concentration
- Conc. Not Determined
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of peripherin in cell and tissue lysates using a peripherin polyclonal antibody (Product # PA1-10012) at a dilution of 1:10,000 as seen in green. 1) protein standard (red), 2) rat spinal cord tissue, 3) mouse spinal cord tissue, 4) pig spinal cord tissue, 5) cow spinal cord; 6) SH-SY5Y, and 7) PC12 cells. The band at 57kDa corresponds to peripherin protein.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Peripherin in mixed fibroblast and PC12 pheochromocytoma cell culture. Samples were stained using a Peripherin polyclonal antibody (Product # PA1-10012) at a dilution of 1:5,000 as seen in green, costained using a vimentin polyclonal antibody at a dilution 1:2,000 as seen in red, and with DAPI staining the nuclear DNA in blue. The Peripherin antibody reveals cytoplasmic filamentous staining in the small PC12 cells, while vimentin antibody stains intermediate filaments in the surrounding fibroblastic cells which do not express peripherin.
