PA1-10018

antibody from Invitrogen Antibodies

Targeting: PRPH NEF4, PRPH1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Recommended by provider)

Antibody Data

Product number

PA1-10018

Provider

Invitrogen Antibodies

Product name

Peripherin Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Purifed from natural sources

Description

PA1-10018 was made against full length human peripherin.| Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Reactivity

Human, Mouse, Rat, Bovine, Porcine

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

Conc. Not Determined

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis was performed on whole cell extracts of SH-SY5Y (Lane 1), IMR32 (Lane 2) and SK-N-AS (Lane 3). The blot was probed with Anti-Peripherin Polyclonal Antibody (Product # PA1-10018, 2 µg/ml) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/ml, 1:4000 dilution). 53, 54 kDa bands corresponding to Peripherin was observed across the cell lines tested.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of tissue and cell lysates using Peripherin polyclonal antibody (Product # PA1-10018) at a dilution of 1:10,000 in green: 1) protein standard, 2) rat spinal cord, 3) mouse spinal cord, 4) pig spinal cord, 5) cow spinal cord, 6) SH-SY5Y cells and 7) PC12 cells. The major band at ~57 kDa corresponds to the major peripherin protein isoform, while other bands presumably represent protein products of alternate transcripts of the peripherin gene.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of the widely used rat pheochromocytoma PC 12 cell line stained with Peripherin polyclonal antibody (Product # PA1-10018, 1:2000) in green. The blue is Hoechst staining of nuclear DNA. Peripherin, one of the Class III family of intermediate filament subunit proteins, is a major component of the PC12 cell forming a perinuclear cap, with some filaments visible in the cytoplasm.