H00005630-M02

antibody from Abnova Corporation

Targeting: PRPH NEF4, PRPH1

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

H00005630-M02

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00005630-M02, RRID:AB_1710867

Product name

PRPH monoclonal antibody (M02), clone 3B3

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a partial recombinant PRPH.

Antigen sequence

RHLREYQELLNVKMALDIEIATYRKLLEGEESRIS
VPVHSFASLNIKTTVPEVEPPQDSHSRKTVLIKTI
ETRNGEVVTESQKEQRSELDKSSAHSY


Isotype

IgG

Antibody clone number

3B3

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

PRPH monoclonal antibody (M02), clone 3B3. Western Blot analysis of PRPH expression in IMR-32(Cat # L008V1 ).

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged PRPH is 1 ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescence of monoclonal antibody to PRPH on HeLa cell . [antibody concentration 10 ug/ml]

Validation comment

Immunofluorescence

Protocol

Protocol

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunoperoxidase of monoclonal antibody to PRPH on formalin-fixed paraffin-embedded human placenta. [antibody concentration 3 ug/ml]

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Protocol

Protocol